School of Pharmaceutical Sciences, Chongqing University, Chongqing 401331, China.
School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2020 Nov 1;1156:122307. doi: 10.1016/j.jchromb.2020.122307. Epub 2020 Aug 9.
Molecularly imprinted polymers (MIPs) based on polydatin were prepared by precipitation polymerization method. Synthesis process of MIPs was optimized by discussion of functional monomers, porogens and the molar ratio of template- functional monomer-cross linker. Then, MIPs were prepared with polydatin as the template, 4-vinyl pyridine as the functional monomer, ethylene glycol dimethyl acrylate as the cross linker, acetonitrile as the porogen and the molar ratio of template-monomer-cross linker at 1:10:20. Scanning electron microscopy and Fourier transform infrared spectrometer were used to inspect macroscale and chemical bond of MIPs. Adsorption capability and selectivity of MIPs to polydatin were investigated by carrying out the static, dynamic and selective experiments. The results showed MIPs performed high adsorption ability and selectivity to polydatin, indicating MIPs could be used to separate and enrich polydatin from the complex systems. Finally, MIPs were applied as the adsorbent for isolation and purification of polydatin from the extract of Polygoni Cuspidati Rhizoma et Radix, rats' plasma and urine samples. MIPs were successfully used to separate polydatin from the Polygoni Cuspidati Rhizoma et Radix and recovery ranged from 89.2% to 91.6%. The maximum concentration of polydatin in rats' plasma and urine samples was 2.84 ± 0.0748 µg mL and 2.64 ± 0.485 µg mL, respectively. Moreover, to compare with the MIPs method, organic solvent methods were used to analyze the polydatin in rats' plasma and urine samples. The results illustrated MIPs method was effective and selective for enrichment of polydatin from the medicinal plants and biological samples.
基于虎杖苷的分子印迹聚合物（MIPs）通过沉淀聚合方法制备。通过讨论功能单体、致孔剂和模板-功能单体-交联剂的摩尔比，优化了 MIPs 的合成工艺。然后，以虎杖苷为模板，4-乙烯基吡啶为功能单体，乙二醇二甲基丙烯酸酯为交联剂，乙腈为致孔剂，模板-单体-交联剂的摩尔比为 1:10:20，制备 MIPs。扫描电子显微镜和傅里叶变换红外光谱仪用于检查 MIPs 的宏观和化学键。通过静态、动态和选择性实验研究了 MIPs 对虎杖苷的吸附能力和选择性。结果表明，MIPs 对虎杖苷具有较高的吸附能力和选择性，表明 MIPs 可用于从复杂体系中分离和富集虎杖苷。最后，将 MIPs 用作从虎杖药材提取物、大鼠血浆和尿液样品中分离和纯化虎杖苷的吸附剂。MIPs 成功地用于从虎杖药材中分离虎杖苷，回收率在 89.2%至 91.6%之间。大鼠血浆和尿液样品中虎杖苷的最大浓度分别为 2.84±0.0748μg/mL 和 2.64±0.485μg/mL。此外，与 MIPs 方法相比，有机溶剂方法用于分析大鼠血浆和尿液样品中的虎杖苷。结果表明，MIPs 方法对从药用植物和生物样品中富集虎杖苷是有效和选择性的。
