Govindaraju M, Shekar H S, Sateesha S B, Vasudeva Raju P, Sambasiva Rao K R, Rao K S J, Rajamma A J
Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India.
Department of Pharmacy Practice, KIMS Hospital and Research Center, VIPS, Bangalore, India.
J Pharm Anal. 2013 Oct;3(5):354-359. doi: 10.1016/j.jpha.2013.03.003. Epub 2013 Apr 28.
In this study, we have demonstrated the conformational changes to DNA induced by abnormal interactions of copper using circular dichroism, in combination with UV-absorbance and fluorescence spectroscopy. Results confirm that binding of copper to bases of DNA in chromatin is concentration dependent. Binding efficiency of Cu ions to DNA is increased in proportion to the degree of unwinding of the double helix induced by denaturation. Altered B-DNA conformation will alter the integrity of DNA which may affect the normal process of DNA replication and transcription. Copper induced DNA damage in the brain may cause neurotoxicity and the neuronal cell death and is implicated in Alzheimer's disease and other neurological disorders.
在本研究中，我们利用圆二色性，并结合紫外吸收光谱和荧光光谱，证明了铜的异常相互作用所诱导的DNA构象变化。结果证实，铜与染色质中DNA碱基的结合具有浓度依赖性。铜离子与DNA的结合效率与变性诱导的双螺旋解旋程度成正比。B-DNA构象的改变将改变DNA的完整性，这可能会影响DNA复制和转录的正常过程。铜诱导的大脑DNA损伤可能导致神经毒性和神经元细胞死亡，并与阿尔茨海默病和其他神经疾病有关。
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