Ünver Ramazan, Deveci Figen, Kırkıl Gamze, Telo Selda, Kaman Dilara, Kuluöztürk Mutlu
Department of Chest Diseases, Fırat University Faculty of Medicine, Elazığ, Turkey.
Department of Biochemestry, Fırat University Faculty of Medicine, Elazığ, Turkey.
Turk Thorac J. 2016 Oct;17(4):153-159. doi: 10.5152/TurkThoracJ.2016.003. Epub 2016 Oct 1.
Chronic Obstructive Pulmonary Disease (COPD) is accompanied by increased cellular stress and inflammation. Most of the Heat Shock Proteins (HSPs) have strong cytoprotective effects. The role of HSPs in COPD pathogenesis has not determined completely. We investigated the serum level of HSPs in COPD patients, smokers without COPD and healthy non-smoking controls. Also, we evaluated the relationship of HSPs with various parameters (inflammatory, oxidative, functional status, quality of life) in COPD patients.
The levels of stress protein (HSP27, HSP70, HSP60, HSP90, CyPA), interleukin-6, C-reactive protein and malondialdehyde were measured in 16 healthy non-smoker, 14 smokers without COPD and 50 patients with stable COPD. Pulmonary function tests (PFT) and arterial blood gases parameters were measured. Health Related Quality of Life was evaluated and exercise capacity was measured with 6 minute walking test.
Only HSP27 levels was significantly higher in COPD patients when compared with both healthy non-smoker and smokers without COPD (for both, p< 0.001). There was a weak-moderate negative correlation between serum levels of HSP27 and PFT parameters and between HSP27 levels and PaO. Serum levels of HSP27 showed a weak-moderate positive correlation with symptom, activity and total scores. Subjects evaluated only smokers without COPD and patients with COPD; HSP27 had an area under the curve (AUC) in the receiver operating characteristic (ROC) curve of 0.819 (0.702-0.935; 95% CI; p= 0.000).
Increased serum levels of HSP27 was found in COPD patients and our results showed sensitivity and specificity of serum HSP27 as diagnostic markers for COPD.
慢性阻塞性肺疾病（COPD）伴有细胞应激和炎症增加。大多数热休克蛋白（HSPs）具有强大的细胞保护作用。HSPs在COPD发病机制中的作用尚未完全明确。我们调查了COPD患者、无COPD的吸烟者和健康非吸烟者血清中HSPs的水平。此外，我们评估了COPD患者中HSPs与各种参数（炎症、氧化、功能状态、生活质量）之间的关系。
测定了16名健康非吸烟者、14名无COPD的吸烟者和50名稳定期COPD患者的应激蛋白（HSP27、HSP70、HSP60、HSP90、CyPA）、白细胞介素-6、C反应蛋白和丙二醛水平。进行了肺功能测试（PFT）和动脉血气参数测定。评估了健康相关生活质量，并通过6分钟步行试验测量了运动能力。
与健康非吸烟者和无COPD的吸烟者相比，COPD患者中仅HSP27水平显著升高（两者均为p<0.001）。血清HSP27水平与PFT参数以及HSP27水平与PaO之间存在弱至中度负相关。血清HSP27水平与症状、活动和总分呈弱至中度正相关。仅对无COPD的吸烟者和COPD患者进行评估；HSP27在受试者工作特征（ROC）曲线中的曲线下面积（AUC）为0.819（0.702 - 0.935；95%CI；p = 0.000）。
在COPD患者中发现血清HSP27水平升高，我们的结果显示血清HSP27作为COPD诊断标志物具有敏感性和特异性。
