Christian Doppler Laboratory for Cardiac and Thoracic Diagnosis and Regeneration, Medical University of Vienna, Vienna, Austria.
Clin Chim Acta. 2012 Jul 11;413(13-14):1115-20. doi: 10.1016/j.cca.2012.03.008. Epub 2012 Mar 23.
Lung cancer represents a major healthcare problem. Accordingly, there is an urgent need to identify serum biomarkers for early diagnosis of lung pathology. We have recently described that patients with manifest COPD evidence elevated levels of heat shock proteins (HSPs). Based on these data, we speculated whether HSPs are also increased in patients with diagnosed lung cancer.
Serum levels of HSP27, phospho-HSP27 (pHSP27) and HSP70 in patients with non-small cell lung cancer (NSCLC) diagnosed at an early (stages I-II, n=37) or advanced (stages IIIA-IV, n=72) stage were determined by using ELISA. Healthy smokers (n=24), healthy never-smoker volunteers (n=33) and COPD patients (n=34) according to GOLD classification served as control population.
Serum levels of HSP27 were elevated in patients with NSCLC diagnosed at an early or advanced stage when compared with both healthy control groups (P<0.005 and P<0.0001 respectively). Statistically significant differences were furthermore found between the groups of patients with early vs. advanced stage NSCLC (P=0.0021). Serum levels of HSP70 were also significantly elevated in patients with NSCLC diagnosed at an early or at an advanced stage when compared with either healthy control groups (P=0.0028 and P<0.0001 respectively). In univariate logistic regression models including healthy subjects and patients with NSCLC, HSP70 had an area under the curve (AUC) of 0.779 (P<0.0001) and HSP27 showed an AUC of 0.870 (P<0.0001).
Our data suggest that serum HSP27 levels might serve as a possible tool to discriminate between early and advanced stages NSCLC.
肺癌是一个主要的医疗保健问题。因此，迫切需要确定用于早期诊断肺病理学的血清生物标志物。我们最近描述了患有明显 COPD 的患者具有升高的热休克蛋白 (HSP) 水平。基于这些数据，我们推测 HSP 是否也在诊断为肺癌的患者中增加。
通过 ELISA 测定早期（I-II 期，n=37）或晚期（IIIA-IV 期，n=72）诊断为非小细胞肺癌（NSCLC）的患者的 HSP27、磷酸化 HSP27（pHSP27）和 HSP70 的血清水平。根据 GOLD 分类，健康吸烟者（n=24）、健康从不吸烟者志愿者（n=33）和 COPD 患者（n=34）作为对照人群。
与两个健康对照组相比，早期或晚期诊断为 NSCLC 的患者的 HSP27 血清水平升高（P<0.005 和 P<0.0001 分别）。此外，在早期与晚期 NSCLC 患者组之间还发现了统计学上的显著差异（P=0.0021）。与任何健康对照组相比，早期或晚期诊断为 NSCLC 的患者的 HSP70 血清水平也显着升高（P=0.0028 和 P<0.0001 分别）。在包括健康受试者和 NSCLC 患者的单变量逻辑回归模型中，HSP70 的曲线下面积（AUC）为 0.779（P<0.0001），HSP27 的 AUC 为 0.870（P<0.0001）。
我们的数据表明，血清 HSP27 水平可能是区分 NSCLC 早期和晚期的一种可能工具。
