Feng Q Q, Dong Z Q, Zhou Y, Zhang H, Long C
Bratisl Lek Listy. 2018;119(1):60-65. doi: 10.4149/BLL_2018_012.
In our study, the impact of miR-16-1-3p on cell proliferation and invasion in NSCLC was explored. miR-16-1-3p mimics were transfected to A549 cells for miR-16-1-3p overexpression. qRT- PCR and Western blot were applied to explore the relative expression of mRNA and protein in A549 cells. Furthermore, the cell proliferation capability was determined by MTT assay. Additionally, cell migration and invasion were measured using a scratch assay and transwell assay, respectively. Moreover, TargetScan and luciferase reporter assay was utilized to investigate the target of miR-16-1-3p. The results indicated that miR-16-1-3p was downregulated in NSCLC cells and upregulation of miR-16-1-3p was able to inhibit the expression of TWIST1. In addition, the reduced cell proliferation, inhibited cell migration and invasion were observed in miR-16-1-3p mimic group compared to the negative control group. The luciferase reporter gene showed that TWIST1 was a target of miR-16-1-3p. Therefore, the present study demonstrated that miR-16-1-3p may suppress A549 cell proliferation, migration and invasion by targeting TWIST1. Thus, miR-16-1-3p might play important roles in NSCLC development, which provides a novel aspect for NSCLC investigation (Fig. 6, Ref. 26).
在我们的研究中，探讨了miR-16-1-3p对非小细胞肺癌（NSCLC）细胞增殖和侵袭的影响。将miR-16-1-3p模拟物转染至A549细胞以实现miR-16-1-3p的过表达。应用qRT-PCR和蛋白质免疫印迹法来探究A549细胞中mRNA和蛋白质的相对表达。此外，通过MTT法测定细胞增殖能力。另外，分别使用划痕试验和Transwell试验来检测细胞迁移和侵袭情况。而且，利用TargetScan和荧光素酶报告基因检测法来研究miR-16-1-3p的靶标。结果表明，NSCLC细胞中miR-16-1-3p表达下调，miR-16-1-3p的上调能够抑制TWIST1的表达。此外，与阴性对照组相比，在miR-16-1-3p模拟物组中观察到细胞增殖减少、细胞迁移和侵袭受到抑制。荧光素酶报告基因显示TWIST1是miR-16-1-3p的一个靶标。因此，本研究表明miR-16-1-3p可能通过靶向TWIST1抑制A549细胞的增殖、迁移和侵袭。因此，miR-16-1-3p可能在NSCLC的发展中发挥重要作用，这为NSCLC的研究提供了一个新的视角（图6，参考文献26）。
