Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, China.
Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, China.
Mater Sci Eng C Mater Biol Appl. 2018 Apr 1;85:170-181. doi: 10.1016/j.msec.2017.12.030. Epub 2017 Dec 29.
Here, we reported a fast, low-cost, and effective fabrication method of large-area and rough halloysite nanotubes (HNTs) coatings by thermal spraying of HNTs ethanol dispersions. A uniform HNTs coating with high transparence is achieved with tailorable surface roughness and thickness. Compared with normal cells, the tumor cells can be captured effectively with high capture yield by the HNTs coatings (expect HeLa cells), which is attributed to the enhanced topographic interactions between HNTs coating and cancer cells. HNTs coating formed from 2.5% ethanol dispersions shows the highest tumor cells capture yeild (90%), which is related to the appropriate roughness and anti-EpCAM conjugation. The capture yield of HNTs coating towards MCF-7 cells can be further improved to 93% within 2h under dynamic shear using a peristaltic pump. The capture yield increases with the incubation time, and the flow rate with 1.25mL/min leads to the maximum capture yield. The HNTs coatings are also effective for capture of tumor cells spiked in artificial blood samples and blood samples from patients with metastatic breast cancer. More than 90% targeted MCF-7 cells and very small amounts of white blood cells are captured by the anti-EpCAM conjugated HNTs coatings from a blood sample. HNTs are further loaded anticancer drug doxorubicin (DOX) and then thermally sprayed into coatings. The MCF-7 cells captured on DOX loaded HNTs coating display significant membrane rupture characteristic and only 3% cell viability after 16h. The high capture efficiency of tumor cells by HNTs coating fabricated by the thermal spraying method makes them show promising applications in clinical circulating tumor cells capture for early diagnosis and monitoring of cancer patients. The high killing ability of the DOX loaded HNTs coating can also be designed as an implantable therapeutic device for preventing tumor metastasis.
在这里，我们报告了一种快速、低成本且有效的大面积和粗糙海泡石纳米管 (HNTs) 涂层制造方法，即通过 HNTs 乙醇分散体的热喷涂。可以实现具有可定制表面粗糙度和厚度的均匀 HNTs 涂层，具有高透明度。与正常细胞相比，肿瘤细胞可以通过 HNTs 涂层有效捕获，捕获率高（除了 HeLa 细胞），这归因于 HNTs 涂层与癌细胞之间增强的形貌相互作用。由 2.5%乙醇分散体制成的 HNTs 涂层显示出最高的肿瘤细胞捕获率（90%），这与适当的粗糙度和抗 EpCAM 缀合有关。使用蠕动泵在动态剪切下，2.5%乙醇分散体制成的 HNTs 涂层在 2 小时内可将 MCF-7 细胞的捕获率进一步提高到 93%。捕获率随孵育时间增加而增加，而流速为 1.25mL/min 时可获得最大捕获率。HNTs 涂层也可有效捕获人工血液样本和转移性乳腺癌患者血液样本中掺入的肿瘤细胞。通过抗 EpCAM 偶联的 HNTs 涂层从血液样本中捕获超过 90%的靶向 MCF-7 细胞和非常少量的白细胞。海泡石进一步负载抗癌药物阿霉素 (DOX)，然后热喷涂成涂层。在负载 DOX 的 HNTs 涂层上捕获的 MCF-7 细胞显示出明显的膜破裂特征，16 小时后细胞活力仅为 3%。通过热喷涂方法制备的 HNTs 涂层对肿瘤细胞的高捕获效率使其在临床循环肿瘤细胞捕获中具有早期诊断和监测癌症患者的应用前景。负载 DOX 的 HNTs 涂层的高杀伤能力也可以设计为用于预防肿瘤转移的植入式治疗装置。
