Groenewegen G, Buurman W A, Jeunhomme G M
Transplantation. 1986 Jun;41(6):751-4. doi: 10.1097/00007890-198606000-00017.
Presentation of autologous alloantigen by certain cells in an allograft can result in allograft rejection. The precise type of cell in a graft responsible for this aspect of allograft rejection remains to be established. Here we report the capacity of canine venous endothelial cells to activate, in vitro, allogeneic lymphocytes for proliferation and differentiation by presentation of alloantigen. Antigen-presenting cell (APC)-depleted lymphocyte populations, prepared in a multistep procedure and tested for absence of APC, were cocultured with allogeneic venous endothelial cells. Proliferation and differentiation into cytotoxic T lymphocytes were measured. While mixed lymphocyte culture of APC-depleted lymphocytes did not result in proliferation and differentiation, coculture of allogeneic APC-depleted lymphocytes with venous endothelial cells resulted in proliferation and generation of cell-mediated cytotoxicity in these cultures. It is concluded that canine venous endothelial cells in vitro have the capacity to present alloantigen. The data suggest an essential role for endothelium in the initial phase of allograft rejection.
同种异体移植物中的某些细胞呈递自身同种异体抗原可导致同种异体移植物排斥反应。移植物中负责同种异体移植物排斥反应这一方面的具体细胞类型仍有待确定。在此，我们报告犬静脉内皮细胞在体外通过呈递同种异体抗原激活同种异体淋巴细胞进行增殖和分化的能力。通过多步骤制备并检测无抗原呈递细胞（APC）的APC缺失淋巴细胞群体，与同种异体静脉内皮细胞共培养。检测增殖情况以及向细胞毒性T淋巴细胞的分化情况。虽然APC缺失淋巴细胞的混合淋巴细胞培养未导致增殖和分化，但同种异体APC缺失淋巴细胞与静脉内皮细胞的共培养导致了这些培养物中的增殖以及细胞介导的细胞毒性的产生。得出的结论是，犬静脉内皮细胞在体外具有呈递同种异体抗原的能力。数据表明内皮细胞在同种异体移植物排斥反应的初始阶段起重要作用。
