Groenewegen G, Buurman W A, Jeunhomme G M, van der Linden C J, Vegt P A, Kootstra G
Transplantation. 1984 Feb;37(2):206-10. doi: 10.1097/00007890-198402000-00017.
Data are presented on the ability of arterial and venous endothelial cells to stimulate allogeneic leukocytes. Mixed cultures of allogeneic endothelium and lymphocytes result in proliferation of lymphocytes and generation of cell-mediated cytotoxicity, which do not occur in cultures of syngeneic combinations of endothelium and lymphocytes. Studies of kinetics showed a peak in proliferation at days 6-7. The optimal responder-stimulator ratio appeared to be 15:1. Lymphocytes stimulated with venous endothelial cells were cytotoxic both for arterial and for venous endothelial cells and PHA blasts of the stimulator dog, whereas lymphocytes stimulated with arterial endothelial cells lysed only arterial endothelial cells and PHA blasts of the stimulator. Lysis of syngeneic or third-party allogeneic control targets was virtually absent. Optimal conditions for long-term culture of effector cells from mixed leukocyte endothelial cell cultures were analyzed. Addition of Il 2 every 3 days and the original stimulating antigen every 6 days permitted continuous proliferation of these cytotoxic lymphocytes with preservation of the cytotoxicity pattern.
本文展示了动脉和静脉内皮细胞刺激同种异体白细胞的能力。同种异体内皮细胞与淋巴细胞的混合培养导致淋巴细胞增殖并产生细胞介导的细胞毒性，而在内皮细胞与淋巴细胞的同基因组合培养中则不会出现这种情况。动力学研究表明，增殖高峰出现在第6 - 7天。最佳反应者与刺激者比例似乎为15:1。用静脉内皮细胞刺激的淋巴细胞对动脉和静脉内皮细胞以及刺激犬的PHA母细胞具有细胞毒性，而用动脉内皮细胞刺激的淋巴细胞仅裂解动脉内皮细胞和刺激者的PHA母细胞。同基因或第三方同种异体对照靶标的裂解几乎不存在。分析了混合白细胞内皮细胞培养物中效应细胞长期培养的最佳条件。每3天添加一次白细胞介素2，每6天添加一次原始刺激抗原，可使这些细胞毒性淋巴细胞持续增殖，并保持细胞毒性模式。
