Groenewegen G, Buurman W A
Scand J Immunol. 1984 Mar;19(3):269-73. doi: 10.1111/j.1365-3083.1984.tb00929.x.
Antigen-presenting cells (APC) were removed from canine peripheral blood by carbonyl iron treatment and adherence to plastic and to nylon-wool. This treatment resulted in low proliferation in mixed lymphocyte cultures (MLC) and lack of generation of cell-mediated cytotoxicity (CMC) in depleted cell suspensions compared with untreated cell suspension. The proliferative response could be restored to normal by the addition of low numbers of autologous arterial or venous endothelial cells to the MLC of depleted cell suspensions. Cytotoxicity against phytohaemagglutinin-stimulated lymphoblasts of the stimulator was generated in the untreated MLC and also in the MLC of APC-depleted cell suspensions with endothelial cells added. It is concluded that arterial and venous endothelial cells can substitute for APC in the proliferative response of autologous lymphocytes against alloantigen and in the generation of CMC. Therefore, endothelial cells have an alloantigen-presenting capacity.
通过羰基铁处理以及贴壁于塑料和尼龙毛，从犬外周血中去除抗原呈递细胞（APC）。与未处理的细胞悬液相比，这种处理导致混合淋巴细胞培养（MLC）中的增殖较低，且在耗尽细胞悬液中缺乏细胞介导的细胞毒性（CMC）的产生。通过向耗尽细胞悬液的MLC中添加少量自体动脉或静脉内皮细胞，增殖反应可恢复正常。在未处理的MLC以及添加了内皮细胞的APC耗尽细胞悬液的MLC中，均产生了针对刺激物的植物血凝素刺激的淋巴母细胞的细胞毒性。结论是，动脉和静脉内皮细胞在自体淋巴细胞对同种异体抗原的增殖反应以及CMC的产生中可以替代APC。因此，内皮细胞具有同种异体抗原呈递能力。
