Ahmad Dar Showkat, Srivastava Prem Prakash, Varghese Tincy, Irfan Rasool Sheikh, Anand Garima, Gupta Subodh, Gireesh-Babu P, Krishna Gopal
Division of Fish Nutrition, Biochemistry and Physiology, ICAR-Central Institute of Fisheries Education, Mumbai 400061, India.
Division of Fish Nutrition, Biochemistry and Physiology, ICAR-Central Institute of Fisheries Education, Mumbai 400061, India.
Gen Comp Endocrinol. 2018 May 15;261:89-96. doi: 10.1016/j.ygcen.2018.01.029. Epub 2018 Jan 31.
A study was carried out to assess the regulation of compensatory growth under different restriction feeding regimes in Labeo rohita juveniles by the interaction of various feed intake and growth regulating genes. A 60 day feeding trial was conducted with five treatment groups, Control (3% body weight, bw), T1 (alternate days), T2 (0.5% bw), T3 (1% bw) and T4 (2% bw) and feeding was done for first 30 days of the trial. For next 30 days, all the treatment groups were fed at a rate of 3% bw as in the control group. There was significant (p < 0.05) difference in the weight gain among the treatment groups with lowest FCR and highest PER was found in T2 group. Ghrelin gene mRNA levels were upregulated during first 30th days of the trial with highest expression levels in the T2 group. The expression levels of leptin gene mRNA were found significantly different (p < 0.05) among the treatments, which was down-regulated during initial 30 days and upregulated as the experiment progress towards 60th day. The IGF-1 mRNA expression levels were upregulated more in liver compared to the muscle tissue. The results of the study suggest that increased ghrelin levels and decreased leptin levels lead to hyperphagia during the onset of refeeding, which further triggers the compensatory growth in L. rohita. The present study describes the molecular mechanism behind the compensatory growth following a different feed restriction regime in L. rohita which is regulated due to the interaction of different energy homeostasis and growth regulating genes.
开展了一项研究，以评估在不同限饲模式下，通过各种采食量和生长调节基因的相互作用，对露斯塔野鲮幼鱼补偿生长的调控。进行了一项为期60天的饲养试验，设置五个处理组，对照组（3%体重，bw）、T1组（隔日投喂）、T2组（0.5% bw）、T3组（1% bw）和T4组（2% bw），在试验的前30天进行投喂。在接下来的30天里，所有处理组均按对照组3% bw的投喂率进行投喂。各处理组间体重增加存在显著差异（p<0.05），T2组的饲料转化率最低，蛋白质效率最高。在试验的前30天，胃饥饿素基因mRNA水平上调，T2组表达水平最高。各处理组间瘦素基因mRNA表达水平存在显著差异（p<0.05），在试验开始的30天内下调，随着试验进行至第60天而上调。与肌肉组织相比，肝脏中IGF-1 mRNA表达水平上调幅度更大。研究结果表明，再投喂开始时，胃饥饿素水平升高和瘦素水平降低导致摄食过量，进而触发露斯塔野鲮的补偿生长。本研究描述了露斯塔野鲮在不同饲料限制模式后补偿生长背后的分子机制，这是由不同能量稳态和生长调节基因的相互作用所调控的。
