Fish Nutrition, Biochemistry and Physiology Division, ICAR-Central Institute of Fisheries Education, Off Yari Road, Panch Marg, Versova, Mumbai, 400061, India.
Institute of Fisheries Post Graduate Studies, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, OMR Campus, Vaniyanchavadi, Chennai, Tamil Nadu, 603103, India.
Fish Physiol Biochem. 2021 Feb;47(1):49-58. doi: 10.1007/s10695-020-00893-7. Epub 2020 Oct 25.
The present study aims to delineate the effect of exogenous enzyme supplementation of fermented or non-fermented de-oiled rice bran (DORB) on haematology, histology and expression of IGF I gene expression of Labeo rohita. Four test diets, namely, T1 (DORB), T2 (fermented DORB), T3 (DORB+exogenous enzyme) and T4 (fermented DORB+exogenous enzyme) were formulated and fed to the L. rohita for a period of 60 days. The test diets T3 and T4 were supplemented with 0.1 g kg xylanase (16,000 U kg) and 0.1 g kg phytase (500 U kg) enzymes. A total of 120 juveniles of L. rohita (average weight 5.01 ± 0.02 g) were stocked in 12 rectangular tanks with 10 fish per tank in triplicates. At the end of the experiment, haematology, histology and IGF I gene expression of the different groups were analysed. The haemoglobin (Hb) content, RBC count and WBC count of L. rohita varied significantly (p < 0.05) among different groups. T3 diet-fed group had the highest Hb content, RBC count and WBC count as compared to the other groups. The MCV content was significantly (p < 0.05) lower in T3 group. The DORB type, exogenous enzyme supplementation and their interaction have no significant (p > 0.05) effect on MCH, MCHC and lymphocyte content of the cultured fish. The group which were fed T3 diet had significantly (p < 0.05) higher IGF-I gene expression as compared to other groups. The histological examination of liver revealed no pathological alteration of this organ. Similarly, there were no pathological changes observed in intestinal tissue in any dietary treatment group. Based on the findings of the present study, it is concluded that exogenous enzyme supplementation of DORB-based diets improves the physiological status and growth performances of Labeo rohita.
本研究旨在描述添加外源酶对发酵或未发酵脱油米糠（DORB）对罗非鱼血液学、组织学和 IGF I 基因表达的影响。设计了四种试验饲料，即 T1（DORB）、T2（发酵 DORB）、T3（DORB+外源酶）和 T4（发酵 DORB+外源酶），并将其投喂罗非鱼 60 天。T3 和 T4 试验饲料添加了 0.1 g kg 木聚糖酶（16,000 U kg）和 0.1 g kg 植酸酶（500 U kg）。共将 120 尾平均体重为 5.01 ± 0.02 g 的罗非鱼幼鱼分入 12 个长方形水槽中，每个水槽 10 尾，设 3 个重复。试验结束时，分析了不同组别的血液学、组织学和 IGF I 基因表达。罗非鱼的血红蛋白（Hb）含量、红细胞计数和白细胞计数在不同组之间存在显著差异（p < 0.05）。与其他组相比，T3 饲料喂养组的 Hb 含量、红细胞计数和白细胞计数最高。T3 组的 MCV 含量显著降低（p < 0.05）。DORB 类型、外源酶添加及其相互作用对养殖鱼类的 MCH、MCHC 和淋巴细胞含量均无显著影响（p > 0.05）。与其他组相比，T3 饲料喂养组的 IGF-I 基因表达显著升高（p < 0.05）。肝脏组织学检查未发现该器官的病理改变。同样，在任何饮食处理组中，肠组织均未观察到病理变化。基于本研究的结果，可以得出结论，DORB 基饲料中外源酶的添加可改善罗非鱼的生理状态和生长性能。
