Department of Experimental Medicine, School of Medicine, University of Perugia, P. le Lucio Severi 1 Sant'Andrea delle Fratte, 06132, Perugia, Italy.
Department of Surgery and Biomedical Sciences, Sect. of Human Anatomy, Clinical and Forensic, School of Medicine, University of Perugia, P. le Lucio Severi 1 Sant'Andrea delle Fratte, 06132, Perugia, Italy.
Food Chem Toxicol. 2018 Mar;113:154-161. doi: 10.1016/j.fct.2018.01.051. Epub 2018 Jan 31.
Melanoma is a severe form of cancer, resistant to conventional therapies. According to in vitro studies, sulforaphane, a dietary component, has been considered a promising antineoplastic candidate. The present study analyzes the in vitro biological effects of sulforaphane in A375 melanoma cell line with or without the addition of Nerve Growth Factor. For the first time, our results show that a supplementation of Nerve Growth Factor partially reverses the sulforaphane-induced: i) inhibition of cell migration, ii) pro apoptotic changes in cell cycle and iii) modulation of active caspase-3. Furthermore, we report the sulforaphane-induced modulation in the expression of Nerve Growth Factor receptors TrKA and p75NTR, shifting their ratio from pro survival to pro apoptotic. In conclusion, the present study evidences that in vivo the antineoplastic effects of sulforaphane may be reduced by the contemporaneous presence of other biological elements such as Nerve Growth Factor and it contributes to a better definition of the real in vivo potentiality of sulforaphane as antineoplastic candidate.
黑色素瘤是一种对抗传统疗法具有抗性的严重癌症。根据体外研究，萝卜硫素，一种饮食成分，被认为是一种很有前途的抗肿瘤候选物。本研究分析了萝卜硫素在 A375 黑色素瘤细胞系中的体外生物学效应，同时添加或不添加神经生长因子。我们的研究结果首次表明，神经生长因子的补充部分逆转了萝卜硫素诱导的：i）细胞迁移抑制，ii）细胞周期中的促凋亡变化，iii）活性 caspase-3 的调节。此外，我们还报告了萝卜硫素诱导的神经生长因子受体 TrKA 和 p75NTR 表达的调节，将它们的比例从促生存转变为促凋亡。总之，本研究表明，在体内，萝卜硫素的抗肿瘤作用可能会因同时存在其他生物因素（如神经生长因子）而降低，这有助于更好地定义萝卜硫素作为抗肿瘤候选物的实际体内潜力。
