Department of Biological Sciences, Auburn University, Auburn, AL 36849, USA; Department of Biochemistry & Molecular Biophysics, Kansas State University, Manhattan, KS 66506-3902, USA.
Department of Biochemistry & Molecular Biophysics, Kansas State University, Manhattan, KS 66506-3902, USA.
J Control Release. 2018 Mar 10;273:139-146. doi: 10.1016/j.jconrel.2018.01.010. Epub 2018 Feb 6.
Development of new and specific insect pest management methods is critical for overcoming pesticide resistance and collateral off-target killings. Gene silencing by feeding dsRNA to insects shows promise in this area. Here we described the use of a peptide nano-material, branched amphiphilic peptide capsules (BAPCs), that facilitates cellular uptake of dsRNA by insects through feeding. The insect diets included dsRNA with and without complexation with BAPCs. The selected insect species come from two different orders with different feeding mechanisms: Tribolium castaneum and Acyrthosiphon pisum. The gene transcripts tested (BiP and Armet) are part of the unfolded protein response (UPR) and suppressing their translation resulted in lethality. For Acyrthosiphon pisum, ingestion of BiP-dsRNA associated with BAPCs led to the premature death of the aphids (t=4-5days) compared to ingestion of the same amounts of free BiP-dsRNA (t=11-12days). Tribolium castaneum was effectively killed using a combination of BiP-dsRNA and Armet-dsRNA complexed with BAPCs; most dying as larvae or during eclosion (75%). Feeding dsRNA alone resulted in fewer deaths (30%). The results show that complexation of dsRNA with BAPCs enhanced the oral delivery of dsRNA over dsRNA alone.
开发新的、特定的害虫管理方法对于克服农药抗性和非靶标杀伤至关重要。通过向昆虫喂食 dsRNA 来进行基因沉默在这方面显示出了前景。在这里，我们描述了一种肽纳米材料——支化两亲肽胶囊（BAPCs）的应用，它通过喂食促进了昆虫对 dsRNA 的细胞摄取。昆虫饲料中包括与 BAPCs 复合和不复合的 dsRNA。所选的昆虫物种来自两个不同的目，具有不同的取食机制：赤拟谷盗和烟粉虱。测试的基因转录物（BiP 和 Armet）是未折叠蛋白反应（UPR）的一部分，抑制它们的翻译会导致致死。对于烟粉虱，与 BAPCs 复合的 BiP-dsRNA 的摄入导致蚜虫提前死亡（t=4-5 天），而摄入相同量的游离 BiP-dsRNA 则导致蚜虫在 11-12 天后死亡（t=11-12 天）。赤拟谷盗用 BiP-dsRNA 和与 BAPCs 复合的 Armet-dsRNA 的组合有效地被杀死；大多数在幼虫或出蛹时死亡（75%）。单独喂食 dsRNA 导致的死亡较少（30%）。结果表明，dsRNA 与 BAPCs 的复合增强了 dsRNA 的口服递送，优于单独的 dsRNA。
