Department of Chemistry and CSGI, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, Italy.
Department of Chemistry and CSGI, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, Italy.
J Colloid Interface Sci. 2018 Apr 15;516:284-294. doi: 10.1016/j.jcis.2018.01.064. Epub 2018 Feb 6.
Understanding the interaction between nanomaterials and biological interfaces is a key unmet goal that still hampers clinical translation of nanomedicine. Here we investigate and compare non-specific interaction of gold nanoparticles (AuNPs) with synthetic lipid and wild type macrophage membranes. A comprehensive data set was generated by systematically varying the structural and physicochemical properties of the AuNPs (size, shape, charge, surface functionalization) and of the synthetic membranes (composition, fluidity, bending properties and surface charge), which allowed to unveil the matching conditions for the interaction of the AuNPs with macrophage plasma membranes in vitro. This effort directly proved for the first time that synthetic bilayers can be set to mimic and predict with high fidelity key aspects of nanoparticle interaction with macrophage eukaryotic plasma membranes. It then allowed to model the experimental observations according to classical interface thermodynamics and in turn determine the paramount role played by non-specific contributions, primarily electrostatic, Van der Waals and bending energy, in driving nanoparticle-plasma membrane interactions.
了解纳米材料与生物界面之间的相互作用是一个尚未实现的关键目标，这仍然阻碍了纳米医学的临床转化。在这里，我们研究并比较了金纳米粒子（AuNPs）与合成脂质和野生型巨噬细胞膜的非特异性相互作用。通过系统地改变 AuNPs（尺寸、形状、电荷、表面功能化）和合成膜（组成、流动性、弯曲性能和表面电荷）的结构和物理化学性质，生成了一个全面的数据集，这使得我们能够揭示 AuNPs 与体外巨噬细胞质膜相互作用的匹配条件。这一努力首次直接证明了合成双层膜可以被设定为模拟和高度准确地预测纳米颗粒与巨噬细胞真核质膜相互作用的关键方面。它随后允许根据经典界面热力学对实验观察进行建模，并反过来确定非特异性贡献（主要是静电、范德华和弯曲能）在驱动纳米颗粒-质膜相互作用中所起的至关重要的作用。
