Department of Medicine, J N Medical College and Hospital, Aligarh Muslim University, Aligarh, India.
Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India.
Clin Chim Acta. 2018 May;480:65-70. doi: 10.1016/j.cca.2018.01.035. Epub 2018 Feb 20.
Pentraxins are a group of highly conserved acute-phase reactant proteins and play crucial role as modulators of inflammatory processes. Pentraxin 3 (PTX3) is primarily produced and released by vascular cell wall, hence, we attempt to establish the role of PTX3 as a biomarker for Rheumatoid Arthritis (RA) compared to CRP.
Thirty patients having active RA as cases and 30 osteoarthritis (OA) patients as controls were recruited. Paired serum and synovial fluid samples were analysed for concentrations of both PTX3 and CRP by using high sensitivity ELISA kit and ROC curve was plotted.
Concentrations of PTX3 and CRP were significantly higher in RA patient serum (p < 0.0001) as well as in synovial fluid (p < 0.0001) and correlated with disease severity. Upon correlation analysis, positive correlation was found between serum and synovial fluid concentrations of PTX3 and CRP. The diagnostic potential of PTX3 was observed in synovial fluid while combination of PTX3 and CRP showed better sensitivity in serum.
PTX3 found to be sensitive non-invasive indicator of clinical arthritic activity in RA patients when compared to traditional markers like CRP. Combination of PTX3 and CRP could serve as better differential diagnostic markers for RA after validation in larger patient cohort.
五聚素是一组高度保守的急性期反应物蛋白，作为炎症过程的调节剂发挥着关键作用。五聚素 3（PTX3）主要由血管壁细胞产生和释放，因此，我们试图将 PTX3 作为类风湿关节炎（RA）的生物标志物与 CRP 进行比较。
招募了 30 名患有活动期 RA 的患者作为病例组和 30 名骨关节炎（OA）患者作为对照组。使用高灵敏度 ELISA 试剂盒分析了两组患者血清和滑液样本中 PTX3 和 CRP 的浓度，并绘制了 ROC 曲线。
RA 患者血清（p<0.0001）和滑液（p<0.0001）中 PTX3 和 CRP 的浓度均显著升高，并与疾病严重程度相关。相关性分析显示，PTX3 在血清和滑液中的浓度与 CRP 呈正相关。PTX3 在滑液中的诊断潜力，当与 CRP 等传统标志物相比时，PTX3 被发现是 RA 患者临床关节炎活动的敏感非侵入性指标。在更大的患者队列中验证后，PTX3 和 CRP 的联合可能成为 RA 的更好鉴别诊断标志物。
