Department of Chemical and Biological Engineering, South Dakota School of Mines and Technology, Rapid City, SD 57701, USA.
Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109, USA.
Bioresour Technol. 2018 May;255:349-353. doi: 10.1016/j.biortech.2018.01.109. Epub 2018 Feb 4.
Human waste simulants were for the first time converted into biohydrogen by a newly developed anaerobic microbial consortium via thermophilic consolidated bioprocessing. Four different BioH-producing consortia (denoted as C1, C2, C3 and C4) were isolated, and developed using human waste simulants as substrate. The thermophilic consortium C3, which contained Thermoanaerobacterium, Caloribacterium, and Caldanaerobius species as the main constituents, showed the highest BioH production (3.999 mmol/g) from human waste simulants under optimized conditions (pH 7.0 and 60 °C). The consortium C3 also produced significant amounts of BioH (5.732 mmol/g and 2.186 mmol/g) using wastewater and activated sludge, respectively. The developed consortium in this study is a promising candidate for H production in space applications as in situ resource utilization.
首次通过新开发的高温厌氧微生物共混物，利用人类粪便模拟物通过嗜热整合生物处理转化为生物氢。分离出四种不同的 BioH 产生共混物（表示为 C1、C2、C3 和 C4），并使用人类粪便模拟物作为底物进行开发。包含 Thermoanaerobacterium、Caloribacterium 和 Caldanaerobius 属作为主要成分的高温共混物 C3 在优化条件（pH 值 7.0 和 60°C）下显示出最高的 BioH 产量（3.999mmol/g）。该共混物 C3 还分别使用废水和活性污泥产生了大量的 BioH（5.732mmol/g 和 2.186mmol/g）。本研究中开发的共混物是原位资源利用在空间应用中生产 H 的有前途的候选物。
