一种用于结核分枝杆菌分离株药敏试验的高通量方法。
A high throughput methodology for susceptibility testing of Mycobacterium tuberculosis isolates.
作者信息
Rampersad Tashmin, Makume Mantha, Sobia Parveen, Willem Sturm A
机构信息
Department of Infection Prevention and Control, Nelson R Mandela School of Medicine, School of Laboratory of Medicine and Medical Science, College of Health Science, University of KwaZulu-Natal, 719 Umbilo Road, Durban 4001, South Africa.
Department of Infection Prevention and Control, Nelson R Mandela School of Medicine, School of Laboratory of Medicine and Medical Science, College of Health Science, University of KwaZulu-Natal, 719 Umbilo Road, Durban 4001, South Africa.
出版信息
J Microbiol Methods. 2018 Mar;146:64-67. doi: 10.1016/j.mimet.2018.02.001. Epub 2018 Feb 2.
MICs for eleven anti-TB drugs with M. tuberculosis isolates were obtained by means of agar dilution with multi-point inoculation. The results were compared with classic agar dilution and the MTT assay. The multi-point inoculation method was reproducible with all drugs and correlated with classic agar dilution and MTT assay. This methodology can be used for routine breakpoint drug susceptibility testing (DST) and for MIC determination.
通过多点接种琼脂稀释法获得了11种抗结核药物对结核分枝杆菌分离株的最低抑菌浓度（MIC）。将结果与经典琼脂稀释法和MTT法进行了比较。多点接种法对所有药物均具有可重复性，且与经典琼脂稀释法和MTT法相关。该方法可用于常规断点药物敏感性试验（DST）和MIC测定。
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