Key Laboratory of Animal Genetics, Breeding, and Reproduction, Ministry of Agriculture & National Engineering Laboratory for Animal Breeding, College of Animal Sciences and Technology, China Agricultural University, Beijing, 100193, China.
Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
Sci Rep. 2018 Feb 6;8(1):2460. doi: 10.1038/s41598-018-20701-w.
Porcine reproductive and respiratory syndrome virus (PRRSV) is a virus susceptible to antibody dependent enhancement, causing reproductive failures in sows and preweaning mortality of piglets. Modified-live virus (MLV) vaccines are used to control PRRS in swine herds. However, immunized sows and piglets often generate variable antibody levels. This study aimed to detect significant genes and pathways involved in antibody responsiveness of pregnant sows and their offspring post-PRRSV vaccination. RNA sequencing was conducted on peripheral blood-mononuclear cells (PBMCs), which were isolated from pregnant sows and their piglets with high (HA), median (MA), and low (LA) PRRS antibody levels following vaccination. 401 differentially expressed genes (DEGs) were identified in three comparisons (HA versus MA, HA versus LA, and MA versus LA) of sow PBMCs. Two novel pathways (complement and coagulation cascade pathway; and epithelial cell signaling in H. pylori infection pathway) revealed by DEGs in HA versus LA and MA versus LA were involved in chemotactic and proinflammatory responses. TNF-α, CCL4, and NFKBIA genes displayed the same expression trends in subsequent generation post-PRRS-MLV vaccination. Findings of the study suggest that two pathways and TNF-α, CCL4, and NFKBIA could be considered as key pathways and potential candidate genes for PRRSV vaccine responsiveness, respectively.
猪繁殖与呼吸综合征病毒（PRRSV）易发生抗体依赖性增强作用，导致母猪繁殖失败和仔猪断奶前死亡。减毒活疫苗（MLV）被用于控制猪群中的 PRRS。然而，免疫母猪和仔猪通常会产生不同的抗体水平。本研究旨在检测与 PRRSV 疫苗接种后妊娠母猪及其后代抗体反应性相关的重要基因和途径。对母猪及其仔猪的外周血单核细胞（PBMCs）进行 RNA 测序，这些 PBMCs是从高（HA）、中（MA）和低（LA）PRRS 抗体水平的母猪及其仔猪中分离出来的。在母猪 PBMCs 的三个比较（HA 与 MA、HA 与 LA 和 MA 与 LA）中，鉴定出了 401 个差异表达基因（DEGs）。HA 与 LA 和 MA 与 LA 中 DEGs 揭示的两个新途径（补体和凝血级联途径；和幽门螺杆菌感染途径中的上皮细胞信号转导）参与了趋化和促炎反应。TNF-α、CCL4 和 NFKBIA 基因在 PRRS-MLV 疫苗接种后的后续世代中表现出相同的表达趋势。研究结果表明，两个途径和 TNF-α、CCL4 和 NFKBIA 可以分别被认为是 PRRSV 疫苗反应性的关键途径和潜在候选基因。
