Leith H M, Truran P L, Gaskell S J
Biomed Environ Mass Spectrom. 1986 May;13(5):257-61. doi: 10.1002/bms.1200130509.
A procedure for the quantification of progesterone in saliva has been developed, using gas chromatography/mass spectrometry (GC/MS) with selected ion monitoring of the 3-enol heptafluorobutyrate derivative. (2H2)Progesterone is used as internal standard. The detection limit (c. 10 pg ml-1) is sufficiently low to permit the determination of the steroid in the saliva of adolescent girls. Assessment of the procedure indicated good assay linearity and excellent accuracy in the determination of progesterone added to saliva. Comparison of GC/MS and radioimmunoassay data showed good agreement, substantiating earlier immunoassay evidence for the occurrence of episodic increases in salivary progesterone concentrations in perimenarcheal girls.
已开发出一种唾液中孕酮定量分析方法，采用气相色谱/质谱联用（GC/MS）对3-烯醇七氟丁酸酯衍生物进行选择离子监测。（2H2）孕酮用作内标。检测限（约10 pg/ml-1）足够低，能够测定青春期女孩唾液中的该类固醇。该方法评估显示，在测定添加到唾液中的孕酮时具有良好的分析线性和出色的准确性。GC/MS数据与放射免疫分析数据比较显示吻合良好，证实了早期免疫分析证据，即月经初潮前女孩唾液中孕酮浓度会出现间歇性升高。
