双酚A暴露与儿童哮喘中GSTP1基因多态性之间的相互作用
Interactions Between Bisphenol A Exposure and GSTP1 Polymorphisms in Childhood Asthma.
作者信息
Lin Tien Jen, Karmaus Wilfried J J, Chen Mei Lien, Hsu Jiin Chyr, Wang I Jen
机构信息
Department of Neurosurgery, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan.
Graduate Institute of Sports Science, College of Exercise and Health Sciences, National Taiwan Sport University, Taoyuan City, Taiwan.
出版信息
Allergy Asthma Immunol Res. 2018 Mar;10(2):172-179. doi: 10.4168/aair.2018.10.2.172.
PURPOSE
Bisphenol A (BPA) exposure may increase the risk of asthma. Genetic polymorphisms of oxidative stress-related genes, glutathione S-transferases (GSTM1, GSTP1), manganese superoxide dismutase, catalase, myeloperoxidase, and microsomal epoxide hydrolase may be related to BPA exposure. The aim is to evaluate whether oxidative stress genes modulates associations of BPA exposure with asthma.
METHODS
We conducted a case-control study comprised of 126 asthmatic children and 327 controls. Urine Bisphenol A glucuronide (BPAG) levels were measured by ultra-performance liquid chromatography/tandem mass spectrometry, and genetic variants were analyzed by a TaqMan assay. Information on asthma and environmental exposure was collected. Analyses of variance and logistic regressions were performed to determine the association of genotypes and urine BPAG levels with asthma.
RESULTS
BPAG levels were significantly associated with asthma (adjusted odds ratio [aOR], 1.29 per log unit increase in concentration; 95% confidence interval [CI], 1.081.55). Compared to the GG genotype, children with a GSTP1 AA genotype had higher urine BPAG concentrations (geometric mean [standard error], 12.72 [4.16] vs 11.42 [2.82]; P=0.036). In children with high BPAG, the GSTP1 AA genotype was related to a higher odds of asthma than the GG genotype (aOR, 4.84; 95% CI, 1.0223.06).
CONCLUSIONS
GSTP1 variants are associated with urine BPA metabolite levels. Oxidative stress genes may modulate the effect of BPA exposure on asthma.
目的
双酚A（BPA）暴露可能会增加患哮喘的风险。氧化应激相关基因的遗传多态性，如谷胱甘肽S-转移酶（GSTM1、GSTP1）、锰超氧化物歧化酶、过氧化氢酶、髓过氧化物酶和微粒体环氧化物水解酶，可能与BPA暴露有关。本研究旨在评估氧化应激基因是否会调节BPA暴露与哮喘之间的关联。
方法
我们进行了一项病例对照研究，纳入了126名哮喘儿童和327名对照。通过超高效液相色谱/串联质谱法测定尿双酚A葡萄糖醛酸苷（BPAG）水平，并采用TaqMan分析法定性分析基因变异。收集有关哮喘和环境暴露的信息。进行方差分析和逻辑回归分析，以确定基因型和尿BPAG水平与哮喘之间的关联。
结果
BPAG水平与哮喘显著相关（校正比值比[aOR]，浓度每增加一个对数单位为1.29；95%置信区间[CI]，1.08-1.55）。与GG基因型相比，GSTP1 AA基因型的儿童尿BPAG浓度更高（几何平均数[标准误]，12.72[4.16]对11.42[2.82]；P=0.036）。在BPAG水平较高的儿童中，GSTP1 AA基因型与哮喘的发生几率高于GG基因型有关（aOR，4.84；95%CI，1.02-23.06）。
结论
GSTP1变异与尿BPA代谢物水平相关。氧化应激基因可能会调节BPA暴露对哮喘的影响。
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