Sarafoff M, Rabes H M, Dörmer P
Carcinogenesis. 1986 Jul;7(7):1191-6. doi: 10.1093/carcin/7.7.1191.
Single cell DNA cytophotometry of ATPase-deficient putative preneoplastic foci in rat liver, induced by a single dose of N-methyl-N-nitrosourea (25 mg/kg or 50 mg/kg) and subsequent phenobarbital feeding (0.05% in the diet), revealed three different types: the majority of foci consisted of an almost exclusive diploid cell population, others showed a preferential tetraploid pattern and a few large foci contained a mixture of di-, tetra- and octoploid hepatocytes. The results of this selective measurement of individual preneoplastic foci in Feulgen-stained 20-microns-thick cryostat sections indicate that not only diploid hepatocytes are a target for the transforming action of a carcinogen, but also tetraploid cells, and that both initiated cell types are capable of expanding as a homogeneous clone. However, clonal homogeneity appears to be lost when foci enlarge. A few preneoplastic foci, heterogeneous with respect to DNA content and endowed with an increased proliferative potential, may represent cell populations at high risk of further development into malignancy.
对经单剂量N-甲基-N-亚硝基脲（25毫克/千克或50毫克/千克）处理并随后喂饲苯巴比妥（饲料中含0.05%）诱导产生的大鼠肝脏中ATP酶缺乏的假定癌前病灶进行单细胞DNA细胞光度测定，结果显示有三种不同类型：大多数病灶几乎完全由二倍体细胞群体组成，其他病灶呈现出优先的四倍体模式，少数大病灶包含二倍体、四倍体和八倍体肝细胞的混合物。在福尔根染色的20微米厚的低温切片中对单个癌前病灶进行这种选择性测量的结果表明，不仅二倍体肝细胞是致癌物转化作用的靶标，四倍体细胞也是，而且这两种起始细胞类型都能够作为同质克隆进行扩增。然而，当病灶扩大时，克隆同质性似乎会丧失。一些在DNA含量方面异质且具有增加的增殖潜能的癌前病灶，可能代表有进一步发展为恶性肿瘤高风险的细胞群体。
