Department of Pharmaceutical Microbiology at the Hans Knöll Institute, Friedrich Schiller University, Jena, Germany.
Spectroscopy/Imaging, Leibniz Institute of Photonic Technology, Jena, Germany.
J Biophotonics. 2018 Jun;11(6):e201700369. doi: 10.1002/jbio.201700369. Epub 2018 Mar 12.
Basidiomycetes, that is, mushroom-type fungi, are known to produce pigments in response to environmental impacts. As antioxidants with a high level of unsaturation, these compounds can neutralize highly oxidative species. In the event of close contact with other microbes, the enzymatically controlled pigment production is triggered and pigment secretion is generated at the interaction zone. The identification and analysis of these pigments is important to understand the defense mechanism of fungi, which is essential to counteract an uncontrolled spread of harmful species. Usually, a detailed analysis of the pigments is time consuming as it depends on laborious sample preparation and isolation procedures. Furthermore, the applied protocols often influence the chemical integrity of the compound of interest. A possibility to noninvasively investigate the pigmentation is Raman microspectroscopy. The methodology has the potential to analyze the chemical composition of the sample spatially resolved at the interaction zone. After the acquisition of a representative spectroscopic library, the pigment production by basidiomycetes was monitored for during response to different fungi and bacteria. The presented results describe a very efficient noninvasive way of pigment analysis which can be applied with minimal sample preparation.
担子菌，即蘑菇型真菌，已知会对环境影响产生色素。这些化合物作为具有高不饱和度的抗氧化剂，可以中和高度氧化的物质。在与其他微生物密切接触的情况下，会触发酶控色素产生，并在相互作用区域产生色素分泌。鉴定和分析这些色素对于了解真菌的防御机制很重要，这对于对抗有害物种的失控传播至关重要。通常，由于依赖于费力的样品制备和分离程序，因此对这些色素进行详细分析会很耗时。此外，所应用的方案通常会影响感兴趣化合物的化学完整性。一种非侵入性调查色素沉着的可能性是拉曼微光谱学。该方法具有在相互作用区域以空间分辨方式分析样品化学成分的潜力。在获得具有代表性的光谱库后，监测担子菌对不同真菌和细菌的反应中的色素产生情况。所呈现的结果描述了一种非常有效的非侵入性色素分析方法，该方法可以在最小的样品制备下应用。
