皮下注射尼古丁对兔机械加工及阳极氧化种植体骨结合的影响。

The effects of subcutaneous injection of nicotine on osseointegration of machined and anodized implants in rabbits.

作者信息

Linden Maria Salete Sandini, Bittencourt Marcos Eugênio de, Carli João Paulo De, Miyagaki Daniela Cristina, Santos Pâmela Letícia Dos, Paranhos Luiz Renato, Groppo Francisco Carlos, Ramacciato Juliana Cama

机构信息

PhD, Full Professor, Department of Dentistry, Universidade de Passo Fundo (UPF), RS, Brazil. Acquisition, analysis and interpretation of data; manuscript writing; final approval.

PhD, Full Professor, Department of Dentistry, UPF, Passo Fundo-RS, Brazil. Statistical analysis, final approval.

出版信息

Acta Cir Bras. 2018 Jan;33(1):31-39. doi: 10.1590/s0102-865020180010000004.

DOI:10.1590/s0102-865020180010000004

PMID:29412231

Abstract

PURPOSE

To evaluate the influence of subcutaneous injection nicotine in osseointegration process on different implant surfaces.

METHODS

Twenty-two male rabbits were distributed into two groups according to the subcutaneous injections: (1) nicotine 3 mg/day/kg and (2) 0.9 % NaCI 3 mL/day/kg, three times a day; subgroups were then designated-machined and anodized implants were placed in the right and left tibia bones, respectively. The animals were submitted euthanasia after periods of eight weeks to determine nicotine and cotinine levels, alkaline phosphatase and biomechanical analysis.

RESULTS

The plasmatic levels of nicotine and cotinine were 0.5 ± 0.28 ng/mL and 9.5 ± 6.51 ng/mL, respectively. The alkaline phosphatase analyses in blood levels in control group were observed 40.8 ± 11.88 UI/L and 40.75 ± 12.46 UI/L, for the surfaces machined and anodized, respectively. In the test group was observed levels 37.9 ± 4.84 UI/L, for both implant surfaces. No significant differences were observed between control and test groups and between the implant surfaces regarding alkaline phosphatase blood levels. For biomechanics, no significant differences were observed in control group between the machined (25±8.46 Ncm) or anodized (31.2 ± 6.76 Ncm) implants. However, the treatment with nicotine induced higher torque than control in both machined (38.3 ± 13.52 Ncm) and anodized (35.5 ± 14.17 Ncm) implants, with p = 0.0024 and p = 0.0121, respectively.

CONCLUSION

Subcutaneous injection of nicotine following implant insertion didn't have effect on osseointegration, independently from the implant surface.

摘要

目的

评估皮下注射尼古丁对不同种植体表面骨结合过程的影响。

方法

将22只雄性兔子根据皮下注射情况分为两组：（1）尼古丁3毫克/天/千克和（2）0.9%氯化钠3毫升/天/千克，每天三次；然后将亚组分别指定为机械加工和阳极氧化种植体，分别植入右胫骨和左胫骨。八周后对动物实施安乐死，以测定尼古丁和可替宁水平、碱性磷酸酶并进行生物力学分析。

结果

尼古丁和可替宁的血浆水平分别为0.5±0.28纳克/毫升和9.5±6.51纳克/毫升。对照组中，机械加工表面和阳极氧化表面血液中的碱性磷酸酶分析结果分别为40.8±11.88国际单位/升和40.75±12.46国际单位/升。在试验组中，两种种植体表面的水平均为37.9±4.84国际单位/升。在对照组和试验组之间以及种植体表面之间，关于碱性磷酸酶血液水平未观察到显著差异。对于生物力学，对照组中机械加工（25±8.46牛顿厘米）或阳极氧化（31.2±6.76牛顿厘米）种植体之间未观察到显著差异。然而，尼古丁治疗在机械加工（38.3±13.52牛顿厘米）和阳极氧化（35.5±14.17牛顿厘米）种植体中均诱导出比对照组更高的扭矩，p值分别为0.0024和0.0121。