Department of Periodontology, School of Dentistry, Chonbuk National University, Jeonju, Jeonbuk, South Korea.
J Periodontol. 2011 Jul;82(7):1035-42. doi: 10.1902/jop.2010.100608. Epub 2010 Dec 28.
Recent investigations reported that osseointegration of titanium implants can be significantly reinforced with a nanostructure treated with anodic oxidation and heat treatment. This experimental study investigates the effect of bisphosphonates on the nanotubular implant surface in rats.
Thirty-six titanium implants were divided into three groups: 1) machine-turned (MT), 2) anodized and heat-treated (AH), and 3) anodized and heat- and bisphosphonate-treated (AHB) groups. The 36 implants were randomly placed in both tibias of 18 male Wistar rats. After 2 and 4 weeks, the levels of osseointegration of the implants were evaluated by a removal torque test and microcomputerized tomography (μCT). Peri-implant bone tissue on the extracted region was examined for the expression of type I collagen and osteocalcin.
The AHB group showed the highest removal torque at 2 and 4 weeks (13.92 ± 1.51 Ncm and 18.10 ± 2.15 Ncm, respectively) followed, in order, by the AH group (11.63 ± 1.58 Ncm at 2 weeks and 14.80 ± 2.34 Ncm at 4 weeks) and MT group (4.30 ± 0.76 Ncm at 2 weeks and 6.20 ± 1.33 Ncm at 4 weeks) with statistically significant differences between the MT and other two groups at both time points. μCT images also revealed a denser appearance around implants in the AHB group than in the other groups. Levels of type I collagen and osteocalcin expression were similar between the MT and AH groups; however, the values were significantly higher in the AHB group compared to the other groups, which were 220.85% ± 71.09% and 363.04% ± 100.21%, respectively (P <0.05).
Within the limits of this experiment, it was concluded that surface loading with bisphosphonates significantly improved the degree of osseointegration of titanium implants with a nanostructure.
最近的研究报告称，经过阳极氧化和热处理的纳米结构处理可以显著增强钛植入物的骨整合。本实验研究了双膦酸盐对大鼠纳米管状植入物表面的影响。
将 36 个钛植入物分为三组：1）机械加工（MT）组，2）阳极氧化和热处理（AH）组，3）阳极氧化和热处理及双膦酸盐处理（AHB）组。36 个植入物随机放置在 18 只雄性 Wistar 大鼠的双侧胫骨中。2 周和 4 周后，通过去除扭矩试验和微计算机断层扫描（μCT）评估植入物的骨整合水平。对提取区域的种植体周围骨组织进行 I 型胶原和骨钙素的表达检测。
AHB 组在 2 周和 4 周时的去除扭矩最高（分别为 13.92 ± 1.51 Ncm 和 18.10 ± 2.15 Ncm），其次是 AH 组（2 周时为 11.63 ± 1.58 Ncm，4 周时为 14.80 ± 2.34 Ncm）和 MT 组（2 周时为 4.30 ± 0.76 Ncm，4 周时为 6.20 ± 1.33 Ncm），MT 组与其他两组在两个时间点均有统计学差异。μCT 图像也显示 AHB 组植入物周围的外观更密集。MT 和 AH 组之间 I 型胶原和骨钙素的表达水平相似；然而，与其他两组相比，AHB 组的值明显更高，分别为 220.85%±71.09%和 363.04%±100.21%（P<0.05）。
在本实验的范围内，可以得出结论，表面加载双膦酸盐显著提高了具有纳米结构的钛植入物的骨整合程度。
