重组Lc36蛋白在犬内脏利什曼病诊断中的潜在应用。
Potential application of rLc36 protein for diagnosis of canine visceral leishmaniasis.
作者信息
Nogueira Camila Tita, Cistia Mayara Lúcia Del, Urbaczek Ana Carolina, Jusi Márcia Mg, Velásquez Angela Maria Arenas, Machado Rosângela Zacarias, Ferreira Henrique, Henrique-Silva Flávio, Langoni Hélio, Costa Paulo Inácio da, Graminha Márcia As
机构信息
Universidade Estadual Paulista, Instituto de Química, Campus de Araraquara, Araraquara, SP, Brasil.
Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas, Campus de Araraquara, Araraquara, SP, Brasil.
出版信息
Mem Inst Oswaldo Cruz. 2018 Mar;113(3):197-201. doi: 10.1590/0074-02760170171.
Visceral leishmaniasis (VL) is fatal if left untreated. Infected dogs are important reservoirs of the disease, and thus specific identification of infected animals is very important. Several diagnostic tests have been developed for canine VL (CVL); however, these tests show varied specificity and sensitivity. The present study describes the recombinant protein rLc36, expressed by Leishmania infantum, as potential antigen for more sensitive and specific diagnosis of CVL based on an immunoenzymatic assay. The concentration of 1.0 μg/mL of rLc36 enabled differentiation of positive and negative sera and showed a sensitivity of 85% and specificity of 71% (with 95% confidence), with an accuracy of 76%.
内脏利什曼病（VL）若不治疗会致命。受感染的犬类是该疾病的重要宿主，因此对受感染动物进行特异性识别非常重要。已经开发了几种用于犬内脏利什曼病（CVL）的诊断测试；然而，这些测试的特异性和敏感性各不相同。本研究描述了由婴儿利什曼原虫表达的重组蛋白rLc36，作为基于免疫酶测定法对CVL进行更敏感和特异性诊断的潜在抗原。浓度为1.0μg/mL的rLc36能够区分阳性和阴性血清，敏感性为85%，特异性为71%（95%置信度），准确率为76%。
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