Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China.
Department of Chemistry, Université de Montréal, C.P. 6128 Succ. Centre-Ville, Montreal, Quebec, Canada H3C 3J7.
Biosens Bioelectron. 2018 May 15;105:226-235. doi: 10.1016/j.bios.2018.01.045.
Biosensors based on converting the concentration of analytes in complex samples into single electrochemical signals are attractive candidates as low cost, high-throughput, portable and renewable sensor platforms. Here, we describe a simple but practical analytical device for sensing an anticancer drug in whole blood, using the detection of methotrexate (MTX) as a model system. In this biosensor, a novel carbon-based composite, tungsten phosphide embedded nitrogen-doped carbon nanotubes (WP/N-CNT), was fixed to the electrode surface that supported redox cycling. The electronic transmission channel in nitrogen doped carbon nanotubes (N-CNT) and the synergistic effect of uniform distribution tungsten phosphide (WP) ensured that the electrode materials have outstanding electrical conductivity and catalytic performance. Meanwhile, the surface electronic structure also endows its surprisingly reproducible performance. To demonstrate portable operation for MTX sensing, screen printing electrodes (SPE) was modified with WP/N-CNT. The sensor exhibited low detection limits (45 nM), wide detection range (0.01-540 μM), good selectivity and long-term stability for the determination of MTX. In addition, the technique was successfully applied for the determination of MTX in whole blood.
基于将复杂样品中的分析物浓度转化为单个电化学信号的生物传感器，作为低成本、高通量、便携式和可再生传感器平台具有吸引力。在这里，我们描述了一种简单但实用的分析装置，用于检测全血中的抗癌药物，以检测甲氨蝶呤 (MTX) 作为模型系统。在该生物传感器中，将一种新型的基于碳的复合材料，即磷钨嵌入氮掺杂碳纳米管 (WP/N-CNT)，固定在支持氧化还原循环的电极表面上。氮掺杂碳纳米管 (N-CNT) 中的电子传输通道和均匀分布的磷钨 (WP) 的协同作用确保了电极材料具有出色的导电性和催化性能。同时，表面电子结构也赋予了其惊人的重现性能。为了证明 MTX 传感的便携操作，对丝网印刷电极 (SPE) 进行了 WP/N-CNT 修饰。该传感器对 MTX 的检测具有低检测限 (45 nM)、宽检测范围 (0.01-540 μM)、良好的选择性和长期稳定性。此外，该技术成功地应用于全血中 MTX 的测定。
