Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, 200237, Shanghai, China.
College of Materials Science & Engineering, Nanjing Tech. University, Nanjing, 210009, China.
Colloids Surf B Biointerfaces. 2018 Apr 1;164:347-357. doi: 10.1016/j.colsurfb.2018.01.058. Epub 2018 Jan 31.
Poor osteogenesis and bacterial infection lead to the failure of implants, thus enhancements of osteogenic activity and antibacterial activity of the implants have significances in orthopedic applications. In this study, macro-microporous bone implants of nano-bioglass (nBG) and polyetheretherketone (PK) composite (mBPC) were fabricated. The results indicated that the mBPC with the porosity of around 70% exhibited interconnected macropores (sizes of about 400 μm) and micropores (sizes of about 10 μm). The apatite mineralization ability of mBPC in simulated body fluid (SBF) was significantly improved compared with macroporous nBG/PK composite (BPC) without micropores and macroporous PK (mPK). Drug of hinokitiol (HK) was loaded into mBPC (dmBPC), which displayed excellent in vitro antibacterial activity against Staphylococcus aureus. The MC3T3-E1 cells proliferation and ALP activity were significantly promoted by mBPC and dmBPC as compared with BPC and mPK. The micro-CT and histological evaluation showed that both mBPC and dmBPC containing nBG and micropores induced higher new bone formation into porous implants than mPK and BPC. The immunohistochemistry analysis indicated that the expression of BMP-2 in mBPC and dmBPC exhibited obviously higher level than mPK and BPC. The results suggested that the incorporation of nBG and micropores in mBPC obviously improved the osteogenic activity, and mBPC load with HK also promoted osteogenesis, indicating good biocompatibility. The dmBPC with HK significantly enhanced osteogenesis and antibacterial activity, which had great potential as bone implant for hard tissue repair.
成骨能力差和细菌感染导致植入物失败，因此增强植入物的成骨活性和抗菌活性在骨科应用中具有重要意义。本研究制备了纳米生物玻璃（nBG）和聚醚醚酮（PK）复合的大-微孔骨植入物（mBPC）。结果表明，具有约 70%孔隙率的 mBPC 具有相互连通的大孔（尺寸约为 400 μm）和微孔（尺寸约为 10 μm）。与没有微孔和大孔 PK（mPK）的大孔 nBG/PK 复合材料（BPC）相比，mBPC 在模拟体液（SBF）中的磷灰石矿化能力显著提高。将 hinokitiol（HK）药物载入 mBPC（dmBPC）中，其对金黄色葡萄球菌的体外抗菌活性表现出优异的效果。与 BPC 和 mPK 相比，MC3T3-E1 细胞的增殖和碱性磷酸酶（ALP）活性在 mBPC 和 dmBPC 上显著提高。微 CT 和组织学评估表明，与 mPK 和 BPC 相比，含有 nBG 和微孔的 mBPC 和 dmBPC 都能诱导更多的新骨形成到多孔植入物中。免疫组织化学分析表明，mBPC 和 dmBPC 中 BMP-2 的表达水平明显高于 mPK 和 BPC。结果表明，mBPC 中加入 nBG 和微孔明显提高了成骨活性，并且负载 HK 的 mBPC 也促进了成骨，表明具有良好的生物相容性。载有 HK 的 dmBPC 显著增强了成骨和抗菌活性，具有作为硬组织修复用骨植入物的巨大潜力。
