基于超薄凹面 Ag 纳米片的用于检测活细胞中释放的 HO 的灵敏电化学无酶生物传感器。
A sensitive electrochemical nonenzymatic biosensor for the detection of HO released from living cells based on ultrathin concave Ag nanosheets.
机构信息
School of Science, MOE Key Laboratory for Non-Equilibrium Synthesis and Modulation of Condensed Matter, State Key Laboratory for Mechanical Behavior of Materials Xi'an Jiaotong University, Xi'an 710049, Shaanxi, China.
School of Science, MOE Key Laboratory for Non-Equilibrium Synthesis and Modulation of Condensed Matter, State Key Laboratory for Mechanical Behavior of Materials Xi'an Jiaotong University, Xi'an 710049, Shaanxi, China; Collaborative Innovation Center of Suzhou Nano Science and Technology, Xi'an Jiaotong University Suzhou Institute, Suzhou 21500, China.
出版信息
Biosens Bioelectron. 2018 May 30;106:29-36. doi: 10.1016/j.bios.2018.01.041. Epub 2018 Jan 31.
Facile synthesis of ultrathin two-dimensional metallic nanosheets with special physical and chemical properties is still challenging. In this work, ultrathin silver nanosheets were synthesized through the galvanic replacement reaction between silver nitrate and Cu microcages without using any surfactant at room temperature. The as-prepared Ag nanosheets (NSs) were concave and had a thickness of sub-10 nm. And the nanosheets exhibited excellent HO detection property with a high sensitivity of about 320.3 uA mM cm and a low detection limit of 0.17 µM in a wide linear range of 5-6000 μM, and a fast response time (less than 2 s). Besides, the real-time detection of HO induced from living HeLa and SH-SY5Y cells were also achieved, indicating the potential application of as-prepared Ag NSs in monitoring physiological and dynamic in-clinic pathological processes.
具有特殊物理化学性质的超薄二维金属纳米片的简便合成仍然具有挑战性。在这项工作中，通过室温下硝酸银和 Cu 微笼之间的电替换反应，在没有使用任何表面活性剂的情况下合成了超薄银纳米片。所制备的 Ag 纳米片（NSs）呈凹形，厚度小于 10nm。并且纳米片表现出优异的 HO 检测性能，在 5-6000µM 的宽线性范围内，灵敏度约为 320.3µA•mM cm，检测限低至 0.17µM，响应时间快（小于 2s）。此外，还实现了对来自活 HeLa 和 SH-SY5Y 细胞的 HO 的实时检测，表明所制备的 Ag NSs 在监测生理和动态临床病理过程方面具有潜在应用。
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