Department of Civil and Environmental Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
Department of Civil and Environmental Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
Chemosphere. 2023 Nov;341:139967. doi: 10.1016/j.chemosphere.2023.139967. Epub 2023 Aug 25.
Activated sludge contains a versatile microbiome capable of converting wastes into valuable chemicals like polyhydroxyalkanoates (PHA). This study investigated the influence of repeated feast and famine phases on PHA production as well as the corresponding microbial population dynamics using waste activated sludge (WAS) as inoculum. Hydrolysate derived from rice straw was employed as a substrate for PHA production. The 16sRNA analysis results revealed that Corynebacteriaceae (40%), Bacillaceae (23%), and Pseudomonas (5%) were the primary contributors to PHA synthesis. Notably, Bacillaceae and Pseudomonas thrived in all the feast and famine phases. The achieved PHA concentration was 3.5 ± 0.2 g/L, and its structure and composition were assessed using Fourier Transform Infrared Spectroscopy (FTIR) and Nuclear Magnetic Resonance (NMR). The analysis revealed that the PHA consists of a copolymer of hydroxybutyrate (HB) and hydroxyvalerate (HV), specifically identified as Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV).
活性污泥中含有一种多功能的微生物群落，能够将废物转化为有价值的化学物质，如聚羟基烷酸酯（PHA）。本研究以废水活性污泥（WAS）为接种物，探讨了反复的饱食和饥饿阶段对 PHA 生产的影响以及相应的微生物种群动态。利用水稻秸秆水解物作为 PHA 生产的底物。16sRNA 分析结果表明，Corynebacteriaceae（40%）、Bacillaceae（23%）和Pseudomonas（5%）是 PHA 合成的主要贡献者。值得注意的是，Bacillaceae 和 Pseudomonas 在所有的饱食和饥饿阶段都大量繁殖。PHA 的浓度达到 3.5±0.2g/L，并使用傅里叶变换红外光谱（FTIR）和核磁共振（NMR）对其结构和组成进行了评估。分析表明，PHA 由羟基丁酸（HB）和羟基戊酸（HV）的共聚物组成，具体鉴定为聚（羟基丁酸-co-羟基戊酸）（PHBV）。
