Immanuel Kant Baltic Federal University, 14 A.Nevskogo St., Kaliningrad 236016, Russia.
Immanuel Kant Baltic Federal University, 14 A.Nevskogo St., Kaliningrad 236016, Russia.
Int Immunopharmacol. 2018 Mar;56:217-221. doi: 10.1016/j.intimp.2018.01.036. Epub 2018 Feb 3.
Interleukin-8 (IL-8, CXCL8) belongs to major chemokines to stimulate migration of neutrophils and monocytes/macrophages (Mc/Mphs) into the inflammation sites. We studied the direct effects of IL-8 on the functionality of human Mc/Mphs in vitro. CD14-positive cells were isolated from human peripheral blood mononuclear cells (PBMCs) by positive magnetic separation and were further cultured with or without lipopolysaccharide (LPS, 1.0 μg/ml) for 24 h. We showed that upon LPS activation of Mc/Mphs, IL-8 reduced markedly both the percentages and median fluorescence intensity (MFI) of CD16 (FcγRIII)-positive cells among CD14 cells, as well as in cells that reduced the expression of СD14 during their culturing. IL-8 was also found to be capable of reducing the expression of СD124 (IL-4 receptor subunit alpha, IL-4RA), with concomitant enhancement of the expression of both CD119 (interferon-gamma receptor 1) and CD197 (CCR7) in Mph cells. In addition, IL-8 up-regulated production of IL-6 and IL-1β [but not tumor necrosis factor-α (TNF-α) and IL-10] by activated Mc/Mphs. Our results suggest the ability for IL-8 to directly favor pro-inflammatory M1-type Mph activity.
白细胞介素-8 (IL-8，CXCL8) 属于主要趋化因子，可刺激中性粒细胞和单核细胞/巨噬细胞 (Mc/Mphs) 迁移到炎症部位。我们研究了白细胞介素-8 对体外人 Mc/Mphs 功能的直接影响。通过正磁分离从人外周血单核细胞 (PBMCs) 中分离出 CD14 阳性细胞，并在有或没有脂多糖 (LPS，1.0μg/ml) 的情况下进一步培养 24 小时。我们表明，在 LPS 激活 Mc/Mphs 后，白细胞介素-8 显著降低了 CD14 细胞中 CD16 (FcγRIII) 阳性细胞的百分比和中位荧光强度 (MFI)，以及在培养过程中降低 CD14 表达的细胞。白细胞介素-8 还能够降低 CD124 (白细胞介素-4 受体亚基 α，IL-4RA) 的表达，同时增强 Mph 细胞中 CD119 (干扰素-γ受体 1) 和 CD197 (CCR7) 的表达。此外，白细胞介素-8 上调了激活的 Mc/Mphs 产生白细胞介素-6 和白细胞介素-1β[但不包括肿瘤坏死因子-α (TNF-α) 和白细胞介素-10]。我们的结果表明，白细胞介素-8 具有直接促进促炎 M1 型 Mph 活性的能力。
