Harbin Institute of Technology, Shenzhen Graduate School, Shenzhen Key Laboratory of Water Resource Utilization and Environmental Pollution Control, Shenzhen 518055, PR China.
College of Energy and Environmental Engineering, Hebei University of Engineering, Handan 056038, PR China.
J Hazard Mater. 2018 May 5;349:10-17. doi: 10.1016/j.jhazmat.2018.01.043. Epub 2018 Feb 6.
Clinically relevant antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in bioaerosols have become a greater threat to public health. However, few reports have shown that ARB and ARGs were found in the atmosphere. High-throughput sequencing applied to environmental sciences has enhanced the exploration of microbial populations in atmospheric samples. Thus, five nosocomial bioaerosols were collected, and the dominant microbial and pathogenic microorganisms were identified by high-throughput sequencing in this study. The results suggested that the dominant microorganisms at the genus level were Massilia, Sphingomonas, Methylobacterium, Methylophilus, Micrococcineae, and Corynebacterineae. The most abundant pathogenic microorganisms were Staphylococcus saprophyticus, Corynebacterium minutissimum, Streptococcus pneumoniae, Escherichia coli, Arcobacter butzleri, Aeromonas veronii, Pseudomonas aeruginosa, and Bacillus cereus. The relationship between microbial communities and environmental factors was evaluated with canonical correspondence analysis (CCA). Meanwhile, differences in the pathogenic bacteria between bioaerosols and dust in a typical hospital was investigated. Furthermore, cultivable Staphylococcus isolates with multi-drug resistance phenotype (>3 antibiotics) in the inpatient departments were much higher than those in the transfusion area and out-patient departments, possibly attributed to the dense usage of antibiotics in inpatient departments. The results of this study might be helpful for scientifically air quality control in hospitals.
临床相关的抗生素耐药细菌（ARB）和抗生素耐药基因（ARGs）在生物气溶胶中对公众健康构成了更大的威胁。然而，很少有报道表明大气中存在 ARB 和 ARGs。高通量测序在环境科学中的应用增强了对大气样本中微生物种群的探索。因此，本研究采集了 5 种医院生物气溶胶，并通过高通量测序鉴定了主要的微生物和致病微生物。结果表明，属水平的主要微生物是马塞利亚菌、鞘氨醇单胞菌、甲基杆菌、甲基单胞菌、微球菌科和棒状杆菌科。最丰富的致病微生物是腐生葡萄球菌、微小棒状杆菌、肺炎链球菌、大肠杆菌、脆弱拟杆菌、维罗纳气单胞菌、铜绿假单胞菌和蜡样芽孢杆菌。采用典范对应分析（CCA）评估了微生物群落与环境因素之间的关系。同时，研究了典型医院生物气溶胶与灰尘中致病菌的差异。此外，住院部耐多药表型（>3 种抗生素）的可培养葡萄球菌分离株明显高于输液区和门诊部，这可能归因于住院部抗生素的密集使用。本研究结果可能有助于对医院空气进行科学质量控制。
