Shahi Maryam, Nadari Mahmood, Sahmani Mehdi, Seyedjafari Ehsan, Ahmadbeigi Naser, Peymani Amir
Department of Molecular Medicine, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.
Cells Tissues Organs. 2018;205(1):9-19. doi: 10.1159/000485122. Epub 2018 Feb 8.
The limitation of traditional bone grafts could be overcome by applying engineered bone constructs, which are mainly produced by seeding suitable stem cells on appropriate scaffolds. So far, bone marrow-derived stromal cells have been the most applied cells in bone tissue engineering, but current data show that unrestricted somatic stem cells (USSCs) from human cord blood might actually be a better stem cell source due to the accessibility and noninvasive procedure of collection. In this study, we cultured USSCs on a plasma-treated electrospun polylactic-co-glycolic acid (PLGA) scaffold coated with nanohydroxyapatite (nHA). Adhesion and proliferation of USSCs on PLGA/nHA were assessed by scanning electron microscopy and MTT assay. Osteogenic differentiation of USSCs into osteoblast lineage cells was evaluated via alkaline phosphatase (ALP) activity and real-time polymerase chain reaction. Our observation showed that USSCs attached and proliferated on PLGA/nHA. Osteogenic differentiation was confirmed by increased ALP activity and OSTEONECTIN expression in USSCs on PLGA/nHA after the 1st week of the osteogenic period. Therefore, using USSCs on electrospun PLGA/nHA is a promising approach in bone tissue engineering.
应用工程化骨构建体可以克服传统骨移植的局限性，工程化骨构建体主要是通过将合适的干细胞接种在合适的支架上制备而成。到目前为止，骨髓来源的基质细胞是骨组织工程中应用最广泛的细胞，但目前的数据表明，由于人脐带血来源的无限制体细胞（USSCs）获取容易且采集过程无创，它们实际上可能是更好的干细胞来源。在本研究中，我们将USSCs培养在经等离子体处理的、涂覆有纳米羟基磷灰石（nHA）的电纺聚乳酸-乙醇酸共聚物（PLGA）支架上。通过扫描电子显微镜和MTT法评估USSCs在PLGA/nHA上的黏附与增殖情况。通过碱性磷酸酶（ALP）活性和实时聚合酶链反应评估USSCs向成骨细胞谱系细胞的成骨分化情况。我们的观察结果显示，USSCs在PLGA/nHA上附着并增殖。在成骨期第1周后，PLGA/nHA上的USSCs中ALP活性增加和骨连接蛋白表达上调，证实了成骨分化。因此，在电纺PLGA/nHA上使用USSCs是骨组织工程中一种有前景的方法。
