Chen Zhuoyue, Song Yue, Zhang Jing, Liu Wei, Cui Jihong, Li Hongmin, Chen Fulin
Lab of Tissue Engineering, Faculty of Life Science, Northwest University, 229 TaiBai North Road, Xi'an, Shaanxi Province 710069, PR China; Provincial Key Laboratory of Biotechnology of Shaanxi, Northwest University, 229 TaiBai North Road, Xi'an, Shaanxi Province 710069, PR China.
Lab of Tissue Engineering, Faculty of Life Science, Northwest University, 229 TaiBai North Road, Xi'an, Shaanxi Province 710069, PR China.
Mater Sci Eng C Mater Biol Appl. 2017 Mar 1;72:341-351. doi: 10.1016/j.msec.2016.11.070. Epub 2016 Nov 24.
Electrospinning is an effective means to generate nano- to micro-scale polymer fibers resembling native extracellular matrix for tissue engineering. However, a major problem of electrospun materials is that limited pore size and porosity may prevent adequate cellular infiltration and tissue ingrowth. In this study, we first prepared thin layers of hydroxyapatite nanoparticle (nHA)/poly-hydroxybutyrate (PHB) via electrospinning. We then laminated the nHA/PHB thin layers to obtain a scaffold for cell seeding and bone tissue engineering. The results demonstrated that the laminated scaffold possessed optimized cell-loading capacity. Bone marrow mesenchymal stem cells (MSCs) exhibited better adherence, proliferation and osteogenic phenotypes on nHA/PHB scaffolds than on PHB scaffolds. Thereafter, we seeded MSCs onto nHA/PHB scaffolds to fabricate bone grafts. Histological observation showed osteoid tissue formation throughout the scaffold, with most of the scaffold absorbed in the specimens 2months after implantation, and blood vessels ingrowth into the graft could be observed in the graft. We concluded that electrospun and laminated nanoscaled biocomposite scaffolds hold great therapeutic potential for bone regeneration.
静电纺丝是一种有效的方法，可生成类似于天然细胞外基质的纳米至微米级聚合物纤维，用于组织工程。然而，静电纺丝材料的一个主要问题是，有限的孔径和孔隙率可能会阻碍细胞充分浸润和组织向内生长。在本研究中，我们首先通过静电纺丝制备了羟基磷灰石纳米颗粒（nHA）/聚羟基丁酸酯（PHB）薄层。然后将nHA/PHB薄层进行层压，以获得用于细胞接种和骨组织工程的支架。结果表明，层压支架具有优化的细胞负载能力。骨髓间充质干细胞（MSCs）在nHA/PHB支架上比在PHB支架上表现出更好的黏附、增殖和成骨表型。此后，我们将MSCs接种到nHA/PHB支架上以制造骨移植物。组织学观察显示，整个支架内有类骨质组织形成，植入2个月后标本中大部分支架被吸收，并且在移植物中可观察到血管长入移植物。我们得出结论，静电纺丝和层压的纳米级生物复合支架在骨再生方面具有巨大的治疗潜力。
