State Key Laboratory of Agricultural Microbiology, National Engineering Research Center of Microbe Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China.
Mar Drugs. 2018 Feb 7;16(2):52. doi: 10.3390/md16020052.
pv. , which causes rice bacterial blight, is one of the most destructive pathogenic bacteria. Biological control against plant pathogens has recently received increasing interest. 1-Deoxy--acetylglucosamine (1-DGlcNAc) was extracted from the supernatant of MCCC 1A00493 fermentation through antibacterial bioassay-guided isolation. Its structure was elucidated by LC/MS, NMR, chemical synthesis and time-dependent density functional theory (TD-DFT) calculations. 1-DGlcNAc specifically suppressed pv. PXO99A (MIC was 23.90 μg/mL), but not other common pathogens including pv. str.8004 and pv. RS105. However, its diastereomer (2-acetamido-1,5-anhydro-2-deoxy-d-mannitol) also has no activity to pv. This result suggested that activity of 1-DGlcNAc was related to the difference in the spatial conformation of the 2-acetamido moiety, which might be attributed to their different interactions with a receptor. Eighty-four unique proteins were found in pv. PXO99A compared with the genome of strains8004 and RS105 by blastp. There may be unique interactions between 1-DGlcNAc and one or more of these unique proteins in pv. . Quantitative real-time PCR and the pharmMapper server indicated that proteins involved in cell division could be the targets in PXO99A. This research suggested that specificity of active substance was based on the active group and spatial conformation selection, and these unique proteins could help to reveal the specific mechanism of action of 1-DGlcNAc against PXO99A.
pv. ，引起水稻细菌性条斑病，是最具破坏性的病原菌之一。生物防治植物病原菌最近受到越来越多的关注。1-脱氧--乙酰氨基葡萄糖（1-DGlcNAc）是从 MCCC 1A00493 发酵液的上清液中通过抗菌生物测定指导分离提取的。通过 LC/MS、NMR、化学合成和时变密度泛函理论（TD-DFT）计算，其结构得到了阐明。1-DGlcNAc 特异性抑制 pv. PXO99A（MIC 为 23.90μg/mL），但对其他常见病原体如 pv. str.8004 和 pv. RS105 则没有活性。然而，其非对映异构体（2-乙酰氨基-1,5-脱水-2-脱氧-d-甘露醇）对 pv. 也没有活性。这一结果表明，1-DGlcNAc 的活性与 2-乙酰氨基部分空间构象的差异有关，这可能归因于它们与受体的不同相互作用。与 8004 株和 RS105 株的基因组相比，通过 blastp 在 pv. PXO99A 中发现了 84 个独特的蛋白质。在 pv. 中，1-DGlcNAc 可能与一个或多个独特蛋白质之间存在独特的相互作用。定量实时 PCR 和 pharmMapper 服务器表明，细胞分裂相关蛋白可能是 PXO99A 的靶标。本研究表明，活性物质的特异性基于活性基团和空间构象选择，这些独特蛋白质有助于揭示 1-DGlcNAc 对 PXO99A 的作用机制。
