State Key Laboratory of Agricultural Microbiology, National Engineering Research Center of Microbe Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China.
Molecules. 2020 Feb 9;25(3):744. doi: 10.3390/molecules25030744.
Root-knot nematode diseases cause severe yield and economic losses each year in global agricultural production. MCCC 1A00493, a deep-sea bacterium, shows a significant nematicidal activity against in vitro. However, information about the active substances of MCCC 1A00493 is limited. In this study, volatile organic compounds (VOCs) from MCCC 1A00493 were isolated and analyzed through solid-phase microextraction and gas chromatography-mass spectrometry. Four VOCs, namely, acetaldehyde, dimethyl disulfide, ethylbenzene, and 2-butanone, were identified, and their nematicidal activities were evaluated. The four VOCs had a variety of active modes on juveniles. Acetaldehyde had direct contact killing, fumigation, and attraction activities; dimethyl disulfide had direct contact killing and attraction activities; ethylbenzene had an attraction activity; and 2-butanone had a repellent activity. Only acetaldehyde had a fumigant activity to inhibit egg hatching. Combining this fumigant activity against eggs and juveniles could be an effective strategy to control the different developmental stages of . The combination of direct contact and attraction activities could also establish trapping and killing strategies against root-knot nematodes. Considering all nematicidal modes or strategies, we could use MCCC 1A00493 to set up an integrated strategy to control root-knot nematodes.
根结线虫病每年在全球农业生产中都会导致严重的产量和经济损失。深海细菌 MCCC 1A00493 对 表现出显著的杀线虫活性。然而，关于 MCCC 1A00493 的活性物质的信息有限。在这项研究中，通过固相微萃取和气相色谱-质谱联用技术对 MCCC 1A00493 的挥发性有机化合物（VOCs）进行了分离和分析。鉴定出 4 种 VOCs，即乙醛、二甲基二硫、乙苯和 2-丁酮，并评估了它们的杀线虫活性。这 4 种 VOCs 对 幼虫有多种活性模式。乙醛具有直接接触致死、熏蒸和引诱活性；二甲基二硫具有直接接触致死和引诱活性；乙苯具有引诱活性；2-丁酮具有驱避活性。只有乙醛具有熏蒸活性可抑制卵孵化。结合这种对卵和幼虫的熏蒸活性可能是控制 不同发育阶段的有效策略。直接接触和引诱活性的结合也可以建立针对根结线虫的诱捕和致死策略。考虑到所有杀线虫模式或策略，我们可以使用 MCCC 1A00493 来建立一种综合策略来控制根结线虫。
