下胚轴中生长素反应、细胞壁pH值和伸长的实时分析
Real-time Analysis of Auxin Response, Cell Wall pH and Elongation in Hypocotyls.
作者信息
Li Lanxin, Krens S F Gabriel, Fendrych Matyáš, Friml Jiří
机构信息
Institute of Science and Technology (IST) Austria, Klosterneuburg, Austria.
出版信息
Bio Protoc. 2018 Jan 5;8(1):e2685. doi: 10.21769/BioProtoc.2685.
Abstract
The rapid auxin-triggered growth of the hypocotyls involves the nuclear TIR1/AFB-Aux/IAA signaling and is accompanied by acidification of the apoplast and cell walls (Fendrych ., 2016). Here, we describe in detail the method for analysis of the elongation and the TIR1/AFB-Aux/IAA-dependent auxin response in hypocotyl segments as well as the determination of relative values of the cell wall pH.
摘要
生长素快速触发的下胚轴生长涉及细胞核TIR1/AFB-Aux/IAA信号传导，并伴随着质外体和细胞壁的酸化（Fendrych等人，2016年）。在这里，我们详细描述了分析下胚轴切段伸长和TIR1/AFB-Aux/IAA依赖性生长素反应的方法，以及测定细胞壁pH相对值的方法。
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