• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

拟南芥 TIR1/AFB 生长素受体的遗传分析揭示了重叠和专门的功能。

Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functions.

机构信息

Section of Cell and Developmental Biology, University of California San Diego, La Jolla, United States.

Plant Molecular and Cellular Biology Laboratory and Integrative Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United States.

出版信息

Elife. 2020 Feb 18;9:e54740. doi: 10.7554/eLife.54740.

DOI:10.7554/eLife.54740
PMID:32067636
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7048394/
Abstract

The TIR1/AFB auxin co-receptors mediate diverse responses to the plant hormone auxin. The Arabidopsis genome encodes six TIR1/AFB proteins representing three of the four clades that were established prior to angiosperm radiation. To determine the role of these proteins in plant development we performed an extensive genetic analysis involving the generation and characterization of all possible multiply-mutant lines. We find that loss of all six TIR1/AFB proteins results in early embryo defects and eventually seed abortion, and yet a single wild-type allele of or is sufficient to support growth throughout development. Our analysis reveals extensive functional overlap between even the most distantly related genes except for . Surprisingly, has a specialized function in rapid auxin-dependent inhibition of root growth and early phase of root gravitropism. This activity may be related to a difference in subcellular localization compared to the other members of the family.

摘要

TIR1/AFB 生长素共受体介导植物激素生长素的多种响应。拟南芥基因组编码六个 TIR1/AFB 蛋白，代表在被子植物辐射之前建立的四个分支中的三个。为了确定这些蛋白质在植物发育中的作用，我们进行了广泛的遗传分析，包括生成和表征所有可能的多重突变系。我们发现，所有六个 TIR1/AFB 蛋白的缺失都会导致早期胚胎缺陷，最终导致种子流产，但单个野生型 或 等位基因足以支持整个发育过程的生长。我们的分析揭示了即使是最遥远相关的 基因之间也存在广泛的功能重叠，但 除外。令人惊讶的是， 在快速依赖生长素的根生长抑制和根向重力性的早期阶段中具有特殊功能。这种活性可能与与家族其他成员相比在亚细胞定位上的差异有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/a8fe59398c38/elife-54740-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/6ccbb5bd733e/elife-54740-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/692a9ef0138d/elife-54740-fig1-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/2c30e3b6a027/elife-54740-fig1-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/f7924186273e/elife-54740-fig1-figsupp3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/fd1610ee39b8/elife-54740-fig1-figsupp4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/0c7f95ad7269/elife-54740-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/696f7dbb153e/elife-54740-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/ec1d691e39e0/elife-54740-fig3-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/4424f8c7b168/elife-54740-fig3-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/0108a0b43ef0/elife-54740-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/d5a5df3c5f55/elife-54740-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/45d82fd94e17/elife-54740-fig5-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/bdd9efbb83c0/elife-54740-fig5-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/7b3ad701d290/elife-54740-fig5-figsupp3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/4d2068de9733/elife-54740-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/f756394932af/elife-54740-fig6-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/66520e1e4b25/elife-54740-fig6-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/f8c5494d0069/elife-54740-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/5f7af128dfa0/elife-54740-fig7-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/a8fe59398c38/elife-54740-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/6ccbb5bd733e/elife-54740-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/692a9ef0138d/elife-54740-fig1-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/2c30e3b6a027/elife-54740-fig1-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/f7924186273e/elife-54740-fig1-figsupp3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/fd1610ee39b8/elife-54740-fig1-figsupp4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/0c7f95ad7269/elife-54740-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/696f7dbb153e/elife-54740-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/ec1d691e39e0/elife-54740-fig3-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/4424f8c7b168/elife-54740-fig3-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/0108a0b43ef0/elife-54740-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/d5a5df3c5f55/elife-54740-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/45d82fd94e17/elife-54740-fig5-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/bdd9efbb83c0/elife-54740-fig5-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/7b3ad701d290/elife-54740-fig5-figsupp3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/4d2068de9733/elife-54740-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/f756394932af/elife-54740-fig6-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/66520e1e4b25/elife-54740-fig6-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/f8c5494d0069/elife-54740-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/5f7af128dfa0/elife-54740-fig7-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afbf/7048394/a8fe59398c38/elife-54740-fig8.jpg

相似文献

1
Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functions.拟南芥 TIR1/AFB 生长素受体的遗传分析揭示了重叠和专门的功能。
Elife. 2020 Feb 18;9:e54740. doi: 10.7554/eLife.54740.
2
Complex regulation of the TIR1/AFB family of auxin receptors.生长素受体 TIR1/AFB 家族的复杂调控。
Proc Natl Acad Sci U S A. 2009 Dec 29;106(52):22540-5. doi: 10.1073/pnas.0911967106. Epub 2009 Dec 16.
3
The E3 ubiquitin ligase SCFTIR1/AFB and membrane sterols play key roles in auxin regulation of endocytosis, recycling, and plasma membrane accumulation of the auxin efflux transporter PIN2 in Arabidopsis thaliana.E3泛素连接酶SCFTIR1/AFB和膜甾醇在拟南芥中生长素对生长素输出载体PIN2的内吞作用、再循环及质膜积累的调控中发挥关键作用。
Plant Cell. 2009 Feb;21(2):568-80. doi: 10.1105/tpc.108.061465. Epub 2009 Feb 13.
4
Plant development is regulated by a family of auxin receptor F box proteins.植物发育受生长素受体F盒蛋白家族调控。
Dev Cell. 2005 Jul;9(1):109-19. doi: 10.1016/j.devcel.2005.05.014.
5
Auxin sensitivities of all Arabidopsis Aux/IAAs for degradation in the presence of every TIR1/AFB.在每种TIR1/AFB存在的情况下，所有拟南芥Aux/IAAs降解的生长素敏感性。
Plant Cell Physiol. 2014 Aug;55(8):1450-9. doi: 10.1093/pcp/pcu077. Epub 2014 May 31.
6
miR393 contributes to the embryogenic transition induced in vitro in Arabidopsis via the modification of the tissue sensitivity to auxin treatment.通过改变组织对生长素处理的敏感性，miR393有助于拟南芥体外诱导的胚胎发生转变。
Planta. 2016 Jul;244(1):231-43. doi: 10.1007/s00425-016-2505-7. Epub 2016 Apr 4.
7
Auxin-binding protein 1 is a negative regulator of the SCF(TIR1/AFB) pathway.生长素结合蛋白 1 是 SCF(TIR1/AFB)途径的负调控因子。
Nat Commun. 2013;4:2496. doi: 10.1038/ncomms3496.
8
SCF(TIR1/AFB)-auxin signalling regulates PIN vacuolar trafficking and auxin fluxes during root gravitropism.SCF(TIR1/AFB)-auxin 信号调节根向重性过程中的 PIN 液泡运输和生长素流。
EMBO J. 2013 Jan 23;32(2):260-74. doi: 10.1038/emboj.2012.310. Epub 2012 Dec 4.
9
The Arabidopsis JAGGED LATERAL ORGANS (JLO) gene sensitizes plants to auxin.拟南芥锯齿状侧器官（JLO）基因使植物对生长素敏感。
J Exp Bot. 2017 May 17;68(11):2741-2755. doi: 10.1093/jxb/erx131.
10
Auxin canalization and vascular tissue formation by TIR1/AFB-mediated auxin signaling in Arabidopsis.拟南芥中通过TIR1/AFB介导的生长素信号传导实现的生长素通道化与维管组织形成
New Phytol. 2020 Jun;226(5):1375-1383. doi: 10.1111/nph.16446. Epub 2020 Feb 22.

引用本文的文献

1
The Evolution of Plant Hormones: From Metabolic Byproducts to Regulatory Hubs.植物激素的演变：从代谢副产物到调控中心
Int J Mol Sci. 2025 Jul 25;26(15):7190. doi: 10.3390/ijms26157190.
2
Sulfur dioxide-induced guard cell death and stomatal closure are attenuated in nitrate/proton antiporter AtCLCa mutants.在硝酸盐/质子反向转运蛋白AtCLCa突变体中，二氧化硫诱导的保卫细胞死亡和气孔关闭受到抑制。
Plant Cell Physiol. 2025 Aug 12;66(7):1076-1085. doi: 10.1093/pcp/pcaf042.
3
"Shape of Cell"-An Auxin and Cell Wall Duet.“细胞形态”——生长素与细胞壁的二重奏。

本文引用的文献

1
One thousand plant transcriptomes and the phylogenomics of green plants.一万种植物转录组与绿色植物的系统发生基因组学
Nature. 2019 Oct;574(7780):679-685. doi: 10.1038/s41586-019-1693-2. Epub 2019 Oct 23.
2
Evolution of fast root gravitropism in seed plants.种子植物快速根向地性的进化。
Nat Commun. 2019 Aug 2;10(1):3480. doi: 10.1038/s41467-019-11471-8.
3
The 35S promoter-driven mDII auxin control sensor is uniformly distributed in leaf primordia.35S 启动子驱动的 mDII 生长素控制传感器均匀分布在叶原基中。
Physiol Plant. 2025 May-Jun;177(3):e70294. doi: 10.1111/ppl.70294.
4
Transcriptional Tuning: How Auxin Strikes Unique Chords in Gene Regulation.转录调控：生长素如何在基因调控中奏响独特乐章
Physiol Plant. 2025 May-Jun;177(3):e70229. doi: 10.1111/ppl.70229.
5
Unresolved roles of Aux/IAA proteins in auxin responses.生长素/吲哚乙酸（Aux/IAA）蛋白在生长素应答中尚未明确的作用。
Physiol Plant. 2025 Mar-Apr;177(2):e70221. doi: 10.1111/ppl.70221.
6
BTB/POZ-MATH proteins regulate Arabidopsis seedling development by promoting auxin-independent degradation of the Aux/IAA protein IAA10.BTB/POZ-MATH蛋白通过促进Aux/IAA蛋白IAA10的非生长素依赖性降解来调节拟南芥幼苗发育。
Plant Physiol. 2025 Apr 30;198(1). doi: 10.1093/plphys/kiaf155.
7
Conserved roles of ETT and ARF4 in gynoecium development in Brassicaceae with distinct fruit shapes.ETT和ARF4在具有不同果实形状的十字花科雌蕊发育中的保守作用。
Development. 2025 Feb 1;152(3). doi: 10.1242/dev.204263. Epub 2025 Feb 12.
8
Role of family genes during grafting in .家族基因在……嫁接过程中的作用
Front Plant Sci. 2024 Nov 22;15:1494579. doi: 10.3389/fpls.2024.1494579. eCollection 2024.
9
Identification of potential auxin response candidate genes for soybean rapid canopy coverage through comparative evolution and expression analysis.通过比较进化和表达分析鉴定大豆快速覆盖冠层的潜在生长素反应候选基因。
Front Plant Sci. 2024 Oct 3;15:1463438. doi: 10.3389/fpls.2024.1463438. eCollection 2024.
10
Genetically Encoded, Noise-Tolerant, Auxin Biosensors in Yeast.酵母中基因编码、抗噪声、生长素生物传感器。
ACS Synth Biol. 2024 Sep 20;13(9):2804-2819. doi: 10.1021/acssynbio.4c00186. Epub 2024 Aug 28.
J Integr Plant Biol. 2019 Nov;61(11):1114-1120. doi: 10.1111/jipb.12853. Epub 2019 Sep 11.
4
Quantitative analysis of auxin sensing in leaf primordia argues against proposed role in regulating leaf dorsoventrality.生长素感应在叶原基中的定量分析否定了其在调节叶片背腹性中的作用。
Elife. 2019 Jan 22;8:e39298. doi: 10.7554/eLife.39298.
5
Something ancient and something neofunctionalized-evolution of land plant hormone signaling pathways.古老与新生：陆地植物激素信号通路的进化
Curr Opin Plant Biol. 2019 Feb;47:64-72. doi: 10.1016/j.pbi.2018.09.009. Epub 2018 Oct 16.
6
Whole-Genome Duplication and Plant Macroevolution.全基因组复制与植物宏观进化。
Trends Plant Sci. 2018 Oct;23(10):933-945. doi: 10.1016/j.tplants.2018.07.006. Epub 2018 Aug 16.
7
Rapid and reversible root growth inhibition by TIR1 auxin signalling.TIR1 生长素信号快速可逆地抑制根生长。
Nat Plants. 2018 Jul;4(7):453-459. doi: 10.1038/s41477-018-0190-1. Epub 2018 Jun 25.
8
Rewiring of auxin signaling under persistent shade.持续遮荫下生长素信号的重新布线。
Proc Natl Acad Sci U S A. 2018 May 22;115(21):5612-5617. doi: 10.1073/pnas.1721110115. Epub 2018 May 3.
9
Origin and evolution of the nuclear auxin response system.核激素响应系统的起源与演化。
Elife. 2018 Mar 27;7:e33399. doi: 10.7554/eLife.33399.
10
Brassicales phylogeny inferred from 72 plastid genes: A reanalysis of the phylogenetic localization of two paleopolyploid events and origin of novel chemical defenses.基于 72 个质体基因推断的 Brassicales 系统发育：对两个古多倍体事件的系统发生定位和新化学防御起源的重新分析。
Am J Bot. 2018 Mar;105(3):463-469. doi: 10.1002/ajb2.1040. Epub 2018 Mar 25.