Malvezzi Helena, Viana Bruno Gallani, Dobo Cristine, Filippi Renee Zon, Podgaec Sérgio, Piccinato Carla Azevedo
Hospital Israelita Albert Einstein, Av. Albert Einstein 627, Morumbi, SP, 05652-900, Brazil.
Arch Gynecol Obstet. 2018 Apr;297(4):977-984. doi: 10.1007/s00404-018-4691-y. Epub 2018 Feb 7.
Endometriosis is a benign disease characterized by implantation and the growth of endometrial tissue outside the uterine cavity and it shares similarities with cancer. Lamin B1, p16 and p21 play a role on cell cycle regulation, development, cell repair and its activities are related to cancers. Considering the similarities between endometriosis and cancer, the aim of the present cross-sectional study is to detect p16, p21 and Lamin B1 in the ectopic endometrium of patients with endometriosis (n = 8) with eutopic (n = 8) and control endometrium (n = 8) and relate them to the maintenance and development of endometriosis.
Biopsies were obtained from both eutopic and ectopic, from deep infiltrating lesions, endometrium frozen and used for immunofluorescent (p16) or immunohistochemistry procedures (p16, p21, lamin B1).
Detected higher lamin B1 in the eutopic endometrium when compared with ectopic endometrium, with no differences between endometriosis tissue with control endometrium. Similar presence of p16 in all groups of patients and no p21 detection was observed.
We observed reduced detection of lamin B1 in the ectopic endometrium raising the possibility that the presence of senescent cells might be contributing to the maintenance and progression of endometriosis by apoptosis resistance and peritoneal stress inherent of the disease.
子宫内膜异位症是一种良性疾病，其特征是子宫内膜组织在子宫腔外植入和生长，且与癌症有相似之处。核纤层蛋白B1、p16和p21在细胞周期调控、发育、细胞修复中发挥作用，其活性与癌症相关。鉴于子宫内膜异位症与癌症的相似性，本横断面研究的目的是检测子宫内膜异位症患者（n = 8）的异位子宫内膜、在位子宫内膜（n = 8）和对照子宫内膜（n = 8）中的p16、p21和核纤层蛋白B1，并将它们与子宫内膜异位症的维持和发展联系起来。
从在位和异位、深部浸润性病变中获取活检组织，将子宫内膜冷冻并用于免疫荧光（p16）或免疫组织化学程序（p16、p21、核纤层蛋白B1）。
与异位子宫内膜相比，在位子宫内膜中检测到更高的核纤层蛋白B1，子宫内膜异位症组织与对照子宫内膜之间无差异。所有患者组中p16的存在情况相似，未观察到p21的表达。
我们观察到异位子宫内膜中核纤层蛋白B1的检测减少，这增加了衰老细胞的存在可能通过抗凋亡和该疾病固有的腹膜应激促进子宫内膜异位症的维持和进展的可能性。
