Hospital Israelita Albert Einstein, Instituto Israelita de Ensino e Pesquisa Albert Einstein, São Paulo, São Paulo, Brazil.
Sci Rep. 2022 Oct 14;12(1):17280. doi: 10.1038/s41598-022-21431-w.
Endometriosis is a prevalent gynecological condition with deleterious effects on women's quality of life in terms of physical, emotional, and social compromise. It is an inflammatory disease characterized by the presence of endometrial-like tissue outside the uterus, and its presentation varies from superficial peritoneal lesions to deep infiltrative endometriosis and ovarian endometrioma. In our previous study, endometriotic lesions were implicated in cellular senescence as their inflammatory pattern could potentially compromise surrounding tissue integrity, thereby inducing a senescent state in cells. P16 and lamin b1 are biomarkers used to assess cellular senescence. Indirect immunofluorescence staining is a broad technique used to assess cellular structure and behavior driven by protein-protein interactions that provide valuable information about cell functioning. The etiopathogeny of endometriosis is not completely understood and diagnostic approaches still rely on invasive methods; therefore, it is important to use validated methods to increase our understanding of the disease and the development of novel diagnostic tools. However, indirect immunofluorescence protocols are often tissue specific and, if neglected, can lead to misinterpretation of results. Moreover, no valid endometriotic tissue-specific colocalization immunofluorescence protocols have been established. Thus, we have validated a well-funded and suitable protocol to allow precise evaluation of the three presentations of endometriosis lesions using indirect immunofluorescence aiming to support further investigations in endometriosis lesions.
子宫内膜异位症是一种常见的妇科疾病，会对女性的生活质量造成身体、情感和社会方面的不良影响。它是一种炎症性疾病，其特征是子宫内膜样组织出现在子宫外，其表现形式从表面腹膜病变到深部浸润性子宫内膜异位症和卵巢子宫内膜异位囊肿不等。在我们之前的研究中，子宫内膜异位症病变与细胞衰老有关，因为其炎症模式可能会损害周围组织的完整性，从而使细胞进入衰老状态。P16 和 lamin b1 是用于评估细胞衰老的生物标志物。间接免疫荧光染色是一种广泛用于评估细胞结构和行为的技术，它由蛋白质-蛋白质相互作用驱动，可以提供有关细胞功能的有价值信息。子宫内膜异位症的病因发病机制尚不完全清楚，诊断方法仍依赖于有创性方法；因此，使用经过验证的方法来提高我们对疾病的认识和开发新的诊断工具非常重要。然而，间接免疫荧光方案通常是针对特定组织的，如果被忽视，可能会导致结果的误解。此外，尚未建立有效的子宫内膜异位症组织特异性共定位免疫荧光方案。因此，我们验证了一种资金充足且合适的方案，以允许使用间接免疫荧光精确评估三种子宫内膜异位症病变的表现，旨在支持子宫内膜异位症病变的进一步研究。
