Institute of Animal Physiology, Centre of Biosciences of the Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia.
University of Veterinary Medicine and Pharmacy, Komenského 73, 048 03, Košice, Slovakia.
Probiotics Antimicrob Proteins. 2018 Sep;10(3):420-426. doi: 10.1007/s12602-018-9390-2.
Probiotic bacteria or their antimicrobial proteinaceous substances called bacteriocins (enterocins) hold promising prophylactic potential for animal breeding. This study present the results achieved after application of Enterocin M in horses. Enterocin M has never been applied to horses before. Clinically healthy horses (10) were involved in this pilot experiment. They were placed in the stables of the University of Veterinary Medicine and Pharmacy, Košice, Slovakia, with the approval of the University Ethics Committee. The animals were fed twice a day with hay and oats, or alternatively grazed with access to water ad libitum. The experiment lasted 6 weeks. Sampling was performed at the start of the experiment, at day 0-1, at day 21 (3 weeks of Enterocin M application), and at day 42 (3 weeks of cessation). Feces were sampled directly from the rectum and blood from the vena jugularis; the samples were immediately treated and/or stored for analyses. Each horse itself represented a control animal (compared to its status at the start of the experiment, day 0-1). After initial sampling, the horses were administered 100 μl of Ent M (precipitate, 12,800 AU/ml) in a small feed bolus to ensure it was consumed; Ent M was applied for 3 weeks (21 days). Fecal samples were treated using the standard microbial dilution method; phagocytic activity was assessed with standard and flow cytometry; biochemistry and metabolic profiles were tested using commercial kits and standard methods. Administration of Ent M led to mathematical reduction of coliforms, campylobacters (P < 0.05), and significant reduction of Clostridium spp. (P < 0.001, P < 0.001); increase of PA values was noted (P < 0.05, P < 0.0001); no negative influence on hydrolytic enzyme profile or biochemical blood parameters was noted.
益生菌或其抗菌蛋白物质，称为细菌素（肠菌素），在动物养殖中具有有希望的预防潜力。本研究介绍了在马中应用肠菌素 M 后获得的结果。肠菌素 M 以前从未在马中应用过。本试验涉及 10 匹临床健康的马。这些马被安置在斯洛伐克考斯卡尼兽医与药学大学的马厩中，得到了大学伦理委员会的批准。这些动物每天喂食两次干草和燕麦，或者自由放牧并可以饮水。试验持续了 6 周。在试验开始时（第 0-1 天）、第 21 天（应用肠菌素 M 3 周）和第 42 天（停止应用 3 周）进行采样。直接从直肠采样粪便，从颈静脉采样血液；立即处理和/或储存样本以进行分析。每匹马本身都是对照动物（与试验开始时相比，第 0-1 天）。初始采样后，将 100μl Ent M（沉淀，12800AU/ml）喂入小马饲料丸中以确保其被消耗；Ent M 应用了 3 周（21 天）。使用标准微生物稀释法处理粪便样本；使用标准和流式细胞术评估吞噬活性；使用商业试剂盒和标准方法测试生化和代谢谱。Ent M 的给药导致大肠菌群、弯曲菌（P<0.05）和梭菌属（P<0.001，P<0.001）的数量数学减少；注意到 PA 值增加（P<0.05，P<0.0001）；未注意到对水解酶谱或生化血液参数有负面影响。
