a Department of BioNano Technology and Gachon BioNano Research Institute , Gachon University , Seongnam , Geonggi-do , Korea.
b Research Institute of Endoderma Co., Ltd , Seongnam , Gyeonggi-do , Korea.
J Drug Target. 2018 Dec;26(10):884-894. doi: 10.1080/1061186X.2018.1435664. Epub 2018 Feb 15.
Microneedles (MN) containing cross-linked hyaluronic acid (X-linked HA) particulates were prepared to control the degradation and swelling behaviour after transdermal drug delivery. The X-linked HA particulates were prepared by cross-linking HA chains and then passing the particulates through a sieve. Then, microneedles were prepared by micromolding method. The rheological properties of X-linked HA were studied. The penetration success rate, mechanical failure and dissolution rate of microneedles containing only hyaluronic acid (HA MN) and microneedles containing X-linked HA were compared. The delivery of fluorescein into the skin with X-linked HA MN was also observed using a confocal microscope. The size of the pulverised particulates in water ranged between 29 and 82 μm in diameter. The HA MN and X-linked HA MN were 270 μm in length. X-linked HA MN with fluorescein was inserted to a depth of 90% of the microneedle length successfully. There was no decrease in the penetration success rate for MN with up to 20% content of X-linked HA particulates. X-linked HA MN with up to 20% of particulate content did not change the dissolution time. Delay in degradation of HA, sustained drug release, and swelling behaviour of the skin layer can be obtained by X-linked HA MN.
制备了含有交联透明质酸（X 型 HA）颗粒的微针，以控制经皮给药后的降解和溶胀行为。X 型 HA 颗粒是通过交联 HA 链，然后将颗粒通过筛子制备的。然后，通过微模塑法制备微针。研究了 X 型 HA 的流变性能。比较了仅含有透明质酸（HA MN）的微针和含有 X 型 HA 的微针的穿透成功率、机械失效和溶解速率。还用共聚焦显微镜观察了 X 型 HA MN 中荧光素递送到皮肤中的情况。水中小粉碎颗粒的直径在 29 到 82μm 之间。HA MN 和 X 型 HA MN 的长度为 270μm。成功地将含有荧光素的 X 型 HA MN 插入到微针长度的 90%深度。含有高达 20%X 型 HA 颗粒的 MN 的穿透成功率没有下降。含有高达 20%颗粒含量的 X 型 HA MN 并没有改变溶解时间。通过 X 型 HA MN 可以获得 HA 的降解延迟、药物持续释放和皮肤层的肿胀行为。
