Zhang Yanxian, Dong Chaofang, Yang Sefei, Chiu Te-Wei, Wu Junsheng, Xiao Kui, Huang Yunhua, Li Xiaogang
1 Corrosion and Protection Center, Key Laboratory for Corrosion and Protection (MOE), 12507 University of Science and Technology, Beijing , China.
2 Department of Stomatology, The PLA General Hospital, Beijing, China.
J Biomater Appl. 2018 Apr;32(9):1289-1299. doi: 10.1177/0885328218755538. Epub 2018 Feb 8.
In this study, we present a novel strategy for hierarchical antibacterial implant coating by controlling structural and componential features as regulators of surface bactericidal property. Anodized titanium dioxide nanotubes and self-polymerized polydopamine were both used as preliminary antibacterial agents with a significant positive effect on surface bioactivity. At the same time, the storage capacity of nanotubes and the in situ reduction activity of polydopamine can introduce large amounts of strong attached silver nanoparticles for enhanced stable antibacterial performance. The surface morphology, chemical composition, and hydrophilicity had been thoroughly characterized. The sustained silver release performances were continuously monitored. The successively in vitro inhibition on Staphylococcus aureus growth of titanium dioxide nanotube, polydopamine layer and silver nanoparticles demonstrated the hierarchical antibacterial property of the final silver nanoparticles-incorporated polydopamine-modified titanium dioxide nanotube coating (silver/polydopamine/nanotube). Moreover, the bioactivity investigation indicated the vital role of polydopamine-modified titanium dioxide nanotube coating on preserving healthy osteoblast activity at the implant interface. The unique hierarchical coating for titanium implant may be a promising method to maximize antibacterial capacity and maintain good cellular activity at the same time.
在本研究中，我们提出了一种通过控制结构和成分特征作为表面杀菌性能调节剂来制备分级抗菌植入物涂层的新策略。阳极氧化二氧化钛纳米管和自聚合聚多巴胺均用作初步抗菌剂，对表面生物活性有显著的积极影响。同时，纳米管的存储能力和聚多巴胺的原位还原活性可引入大量牢固附着的银纳米颗粒，以增强稳定的抗菌性能。对表面形态、化学成分和亲水性进行了全面表征。持续监测银的释放性能。二氧化钛纳米管、聚多巴胺层和银纳米颗粒对金黄色葡萄球菌生长的体外连续抑制作用证明了最终含银纳米颗粒的聚多巴胺改性二氧化钛纳米管涂层（银/聚多巴胺/纳米管）的分级抗菌性能。此外，生物活性研究表明聚多巴胺改性二氧化钛纳米管涂层在植入物界面保持健康成骨细胞活性方面起着至关重要的作用。钛植入物独特的分级涂层可能是一种同时最大化抗菌能力和保持良好细胞活性的有前途的方法。
