曲霉属中微生物次级代谢产物生物合成的重建研究进展
Recent advances in reconstructing microbial secondary metabolites biosynthesis in Aspergillus spp.
机构信息
College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei Province 430023, PR China; Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Wuhan, Hubei Province 430023, PR China; Hubei Key Laboratory for Processing and Transformation of Agricultural Products (Wuhan Polytechnic University), Wuhan, Hubei Province 430023, PR China.
Food College, Shihezi University, Shihezi, Xinjiang, Uygur Autonomous Region 832000, PR China.
出版信息
Biotechnol Adv. 2018 May-Jun;36(3):739-783. doi: 10.1016/j.biotechadv.2018.02.001. Epub 2018 Feb 5.
High throughput genome sequencing has revealed a multitude of potential secondary metabolites biosynthetic pathways that remain cryptic. Pathway reconstruction coupled with genetic engineering via heterologous expression enables discovery of novel compounds, elucidation of biosynthetic pathways, and optimization of product yields. Apart from Escherichia coli and yeast, fungi, especially Aspergillus spp., are well known and efficient heterologous hosts. This review summarizes recent advances in heterologous expression of microbial secondary metabolite biosynthesis in Aspergillus spp. We also discuss the technological challenges and successes in regard to heterologous host selection and DNA assembly behind the reconstruction of microbial secondary metabolite biosynthesis.
高通量基因组测序揭示了大量潜在的次级代谢产物生物合成途径，但这些途径仍然是隐藏的。通过异源表达进行途径重建和遗传工程，能够发现新的化合物、阐明生物合成途径，并优化产物产量。除了大肠杆菌和酵母外，真菌，特别是 Aspergillus 属真菌，是众所周知的高效异源宿主。本文综述了 Aspergillus 属真菌中微生物次级代谢产物生物合成的异源表达的最新进展。我们还讨论了在微生物次级代谢产物生物合成重建中，异源宿主选择和 DNA 组装方面的技术挑战和成功案例。
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