Marucco Andrea P, Minervini Patricia, Snitman Gabriela V, Sorge Adriana, Guelfand Liliana I, Moral Laura López
Hospital de Agudos Santojanni, CABA, Argentina.
Hospital Oftalmológico Santa Lucía, CABA, Argentina.
Rev Argent Microbiol. 2018 Oct-Dec;50(4):337-340. doi: 10.1016/j.ram.2017.10.003.
In patients with invasive fungal infections, the accurate and rapid identification of the genus Candida is of utmost importance since antimycotic sensitivity is closely related to the species. The aim of the present study was to compare the identification results of species of the genus Candida obtained by BD Phoenix™ (Becton Dickinson [BD]) and Maldi-TOF MS (Bruker Microflex LT Biotyper 3.1). A total of 192 isolates from the strain collection belonging to the Mycology Network of the Autonomous City of Buenos Aires, Argentina, were analyzed. The observed concordance was 95%. Only 10 strains (5%) were not correctly identified by the BD Phoenix™ system. The average identification time with the Yeast ID panels was 8h 22min. The BD Phoenix™ system proved to be a simple, reliable and effective method for identifying the main species of the genus Candida.
在侵袭性真菌感染患者中，准确快速地鉴定念珠菌属至关重要，因为抗真菌药敏性与菌种密切相关。本研究的目的是比较BD Phoenix™（美国BD公司）和基质辅助激光解吸电离飞行时间质谱（Maldi-TOF MS，布鲁克Microflex LT Biotyper 3.1）获得的念珠菌属菌种鉴定结果。对来自阿根廷布宜诺斯艾利斯自治市真菌学网络菌株库的192株分离株进行了分析。观察到的一致性为95%。BD Phoenix™系统仅10株（5%）未正确鉴定。使用酵母鉴定板的平均鉴定时间为8小时22分钟。BD Phoenix™系统被证明是鉴定念珠菌属主要菌种的一种简单、可靠且有效的方法。
