基质辅助激光解吸电离技术的一个发霉应用：用于真菌鉴定的基质辅助激光解吸电离飞行时间质谱分析

Department of Laboratory Medicine and Pathology, Division of Clinical Microbiology, Mayo Clinic, Rochester, MN 55905, USA.

Department of Medicine, Mayo Clinic, Division of Infectious Diseases, Rochester, MN 55905, USA.

J Fungi (Basel). 2019 Jan 3;5(1):4. doi: 10.3390/jof5010004.

As a result of its being inexpensive, easy to perform, fast and accurate, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF MS) is quickly becoming the standard means of bacterial identification from cultures in clinical microbiology laboratories. Its adoption for routine identification of yeasts and even dimorphic and filamentous fungi in cultures, while slower, is now being realized, with many of the same benefits as have been recognized on the bacterial side. In this review, the use of MALDI-ToF MS for identification of yeasts, and dimorphic and filamentous fungi grown in culture will be reviewed, with strengths and limitations addressed.

由于基质辅助激光解吸电离飞行时间质谱（MALDI-ToF MS）价格低廉、操作简便、快速且准确，它正迅速成为临床微生物实验室从培养物中鉴定细菌的标准方法。虽然它在培养物中用于酵母甚至双态和丝状真菌的常规鉴定的应用速度较慢，但现在正在实现，并且具有许多与细菌鉴定方面相同的优势。在本综述中，将对MALDI-ToF MS用于鉴定培养物中生长的酵母、双态和丝状真菌的情况进行综述，并讨论其优点和局限性。