Maimaitiaili Abudunaibi, Li Junhong, Aibibula Aili, Abudureheman Mulati
Department of Cardiac Surgery, The First Affiliated of Xinjiang Medical UniversityUrumqi 830011, P. R. China.
Am J Transl Res. 2018 Jan 15;10(1):77-85. eCollection 2018.
Uighur medicine Fufang Munziq granule (Munziq) [corrected] shows cardioprotective effect in myocardial ischemia/reperfusion injury (IRI) in animal models, but the molecular mechanism of this effect is not clear. The present study investigates the regulation of nuclear factor kappa B (NF-kappa B) pathway by Fufang Munziq granule (Munziq) in IRI rat models.
Male Sprague-Dawley rats were divided into three groups: the NF-κB gene knockout group (n = 5); the NF-κB transgenic group (n = 8); and the control group (n = 5). All rats were treated with Fufang Munziq granule (Munziq) [corrected] for 21 days before underwent IRI surgical procedure. Blood and tissue samples were collected for the RT-PCR, ELISA, western blot and other examination.
Expression of NF-κB p65mRNA and protein were down-regulated in the NF-κB knockout rats but up-regulated in the transgenic rats comparing with the controls (P<0.05). The upstream NF-κB kinase expressions, the downstream inflammatory cytokines, and the myocardial injury markers were all changed in accordance with the NF-κB gene modification (all values <0.05). AMSq treatment relieved IRI in the NF-κB knockout rats.
Inhibition on NF-κB signaling pathway may alleviate IRI in rats under Fufang Munziq granule (Munziq) [corrected] treatment.
维吾尔药复方木尼孜其颗粒（木尼孜其）在动物模型的心肌缺血/再灌注损伤（IRI）中显示出心脏保护作用，但其作用的分子机制尚不清楚。本研究探讨复方木尼孜其颗粒（木尼孜其）对IRI大鼠模型中核因子κB（NF-κB）信号通路的调控作用。
将雄性Sprague-Dawley大鼠分为三组：NF-κB基因敲除组（n = 5）；NF-κB转基因组（n = 8）；对照组（n = 5）。所有大鼠在接受IRI手术前均用复方木尼孜其颗粒（木尼孜其）治疗21天。采集血液和组织样本进行RT-PCR、ELISA、western blot等检测。
与对照组相比，NF-κB基因敲除大鼠中NF-κB p65mRNA和蛋白表达下调，而转基因大鼠中表达上调（P<0.05）。上游NF-κB激酶表达、下游炎性细胞因子和心肌损伤标志物均随NF-κB基因改变而变化（所有P值<0.05）。木尼孜其治疗可减轻NF-κB基因敲除大鼠的IRI。
抑制NF-κB信号通路可能减轻复方木尼孜其颗粒（木尼孜其）治疗下大鼠的IRI。
