Migeot Valerie, Hermand Damien
URPHYM-GEMO, The University of Namur, Namur, Belgium.
Methods Mol Biol. 2018;1721:25-34. doi: 10.1007/978-1-4939-7546-4_3.
The distribution of modified histones within the fission yeast Schizosaccharomyces pombe genome is ultimately dependent upon the transcriptional activity and in turn influences the ability of the polymerases to bind and progress through the chromatin template. The Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) method currently provides the highest resolution, accuracy, and reproducibility to characterize histones modifications within a defined region of the genome. The following protocol details the method applied to S. pombe.
修饰组蛋白在裂殖酵母粟酒裂殖酵母基因组中的分布最终取决于转录活性，进而影响聚合酶与染色质模板结合并在其上进行转录的能力。染色质免疫沉淀-聚合酶链反应（ChIP-PCR）方法目前在表征基因组特定区域内的组蛋白修饰方面具有最高的分辨率、准确性和可重复性。以下方案详细介绍了应用于粟酒裂殖酵母的方法。
