裂殖酵母细胞周期中的端粒调控
Telomere regulation during the cell cycle in fission yeast.
作者信息
Moser Bettina A, Chang Ya-Ting, Nakamura Toru M
机构信息
Department of Biochemistry and Molecular Genetics, College of Medicine, University of Illinois at Chicago, 900 S. Ashland (M/C 669), Chicago, IL, 60607, USA.
出版信息
Methods Mol Biol. 2014;1170:411-24. doi: 10.1007/978-1-4939-0888-2_22.
Abstract
The fission yeast Schizosaccharomyces pombe has emerged as a useful model organism to study telomere maintenance mechanisms. In this chapter, we provide detailed protocols for quantitative ChIP and BrdU incorporation analyses to investigate how fission yeast telomeres are regulated during the cell cycle by utilizing cdc25-22 synchronized cell cultures.
摘要
裂殖酵母粟酒裂殖酵母已成为研究端粒维持机制的有用模式生物。在本章中，我们提供了定量染色质免疫沉淀（ChIP）和5-溴脱氧尿嘧啶核苷（BrdU）掺入分析的详细方案，以研究利用cdc25-22同步化细胞培养物时裂殖酵母端粒在细胞周期中是如何被调控的。
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2
Protection and replication of telomeres in fission yeast.裂殖酵母中端粒的保护和复制。
Biochem Cell Biol. 2009 Oct;87(5):747-58. doi: 10.1139/O09-037.
3
Taming the tiger by the tail: modulation of DNA damage responses by telomeres.擒贼先擒王：端粒对 DNA 损伤反应的调节。
EMBO J. 2009 Aug 5;28(15):2174-87. doi: 10.1038/emboj.2009.176. Epub 2009 Jul 23.
4
Differential arrival of leading and lagging strand DNA polymerases at fission yeast telomeres.前导链和后随链DNA聚合酶在裂殖酵母端粒处的差异到达。
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5
Growth and manipulation of S. pombe.粟酒裂殖酵母的生长与操作
Curr Protoc Mol Biol. 2003 Nov;Chapter 13:Unit 13.16. doi: 10.1002/0471142727.mb1316s64.
6
Replication and protection of telomeres.端粒的复制与保护
Nature. 2007 Jun 21;447(7147):924-31. doi: 10.1038/nature05976.
7
Protocol for the fast chromatin immunoprecipitation (ChIP) method.快速染色质免疫沉淀（ChIP）方法的实验方案。
Nat Protoc. 2006;1(1):179-85. doi: 10.1038/nprot.2006.27.
8
Genome-wide localization of pre-RC sites and identification of replication origins in fission yeast.裂殖酵母中前复制复合体（pre-RC）位点的全基因组定位及复制起始点的鉴定。
EMBO J. 2007 Mar 7;26(5):1327-39. doi: 10.1038/sj.emboj.7601585. Epub 2007 Feb 15.
9
Telomere binding protein Taz1 establishes Swi6 heterochromatin independently of RNAi at telomeres.端粒结合蛋白Taz1在端粒处独立于RNA干扰建立Swi6异染色质。
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10
In vivo labeling of fission yeast DNA with thymidine and thymidine analogs.利用胸苷和胸苷类似物对裂殖酵母DNA进行体内标记。
Methods. 2004 Jul;33(3):213-9. doi: 10.1016/j.ymeth.2003.11.016.
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