Moser Bettina A, Chang Ya-Ting, Nakamura Toru M
Department of Biochemistry and Molecular Genetics, College of Medicine, University of Illinois at Chicago, 900 S. Ashland (M/C 669), Chicago, IL, 60607, USA.
Methods Mol Biol. 2014;1170:411-24. doi: 10.1007/978-1-4939-0888-2_22.
The fission yeast Schizosaccharomyces pombe has emerged as a useful model organism to study telomere maintenance mechanisms. In this chapter, we provide detailed protocols for quantitative ChIP and BrdU incorporation analyses to investigate how fission yeast telomeres are regulated during the cell cycle by utilizing cdc25-22 synchronized cell cultures.
裂殖酵母粟酒裂殖酵母已成为研究端粒维持机制的有用模式生物。在本章中，我们提供了定量染色质免疫沉淀（ChIP）和5-溴脱氧尿嘧啶核苷（BrdU）掺入分析的详细方案，以研究利用cdc25-22同步化细胞培养物时裂殖酵母端粒在细胞周期中是如何被调控的。
