Morimoto Noriko, Ueno Kotomi, Teraishi Masayoshi, Okumoto Yutaka, Mori Naoki, Ishihara Atsushi
a Graduate School of Agriculture , Tottori University , Tottori , Japan.
b Faculty of Agriculture , Tottori University , Tottori , Japan.
Biosci Biotechnol Biochem. 2018 Mar;82(3):407-416. doi: 10.1080/09168451.2018.1429889. Epub 2018 Feb 9.
Rice plants accumulate various specialized metabolites, including phenylamides, in response to pathogen attack. We prepared 25 phenylamides, and developed a method of analyzing them by multiple reaction monitoring with liquid chromatography coupled with tandem mass spectrometry. We analyzed phenylamides in rice leaves infected with Cochliobolus miyabeanus and Xanthomonas oryzae. The phenylamides induced included benzoyltryptamine, cinnamoyl-, p-coumaroyl-, feruloyl-, and benzoylserotonins, cinnamoyl and benzoyltyramines, feruloylagmatine, and feruloylputrescine. Some of the phenylamides exhibited antimicrobial activity against C. miyabeanus and X. oryzae, indicating that they are phytoalexins. Treatment with jasmonic acid, salicylic acid, 6-benzylaminopurine, and ethephone also induced phenylamide accumulation. The compositions of the induced amides varied depending on the plant hormone used, and cinnamoyltryptamine, cinnamoylserotonin, and cinnamoyltyramine were not induced by the plant hormones. These findings suggest that several plant hormones and additional factors are involved in phenylamide accumulation in response to pathogen infection in rice.
水稻植株在受到病原体攻击时会积累各种特殊代谢产物，包括苯酰胺。我们制备了25种苯酰胺，并开发了一种通过液相色谱-串联质谱联用的多反应监测来分析它们的方法。我们分析了感染宫部旋孢腔菌和水稻黄单胞菌的水稻叶片中的苯酰胺。诱导产生的苯酰胺包括苯甲酰色胺、肉桂酰-、对香豆酰-、阿魏酰-和苯甲酰血清素、肉桂酰和苯甲酰酪胺、阿魏酰胍丁胺和阿魏酰腐胺。其中一些苯酰胺对宫部旋孢腔菌和水稻黄单胞菌具有抗菌活性，表明它们是植保素。用茉莉酸、水杨酸、6-苄基腺嘌呤和乙烯利处理也会诱导苯酰胺积累。诱导产生的酰胺成分因所用植物激素而异，肉桂酰色胺、肉桂酰血清素和肉桂酰酪胺不会被植物激素诱导产生。这些发现表明，几种植物激素和其他因素参与了水稻对病原体感染的苯酰胺积累反应。
