Otrubová Oľga, Turecký Ladislav, Uličná Oľga, Janega Pavol, Luha Ján, Muchová Jana
Department of Occupational Medicine and Toxicology, National Toxicological Information Centre, University Hospital Bratislava, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
Gen Physiol Biophys. 2018 Jan;37(1):23-31. doi: 10.4149/gpb_2017016.
N-acetyl-L-cysteine (NAC) is a drug routinely used in several health problems, e.g. liver damage. There is some information emerged on its negative effects in certain situations. The aim of our study was to examine its ability to influence liver damage induced by long-term burden. We induced liver damage by CCl4 (10 weeks) and monitored the impact of parallel NAC administration (daily 150 mg/kg of b.w.) on liver morphology and some biochemical parameters (triacylglycerols, cholesterol, alanine aminotransferase (ALT), aspartate aminotransferase (AST), bilirubin, bile acids, proteins, albumins and cholinesterase). NAC significantly decreased levels of bile acids and bilirubin in plasma and triacylglycerols in liver, all of them elevated by impairment with CCl4. Reduction of cholesterol induced by CCl4 was completely recovered in the presence of NAC as indicated by its elevation to control levels. NAC administration did not improve the histological parameters. Together with protective effects of NAC, we found also its deleterious properties: parallel administration of CCl4 and NAC increased triacylglycerols, ALT and AST activity and significantly increased plasma cholinesterase activity. We have observed nonsignificantly increased percentage of liver tissue fibrosis. Our results have shown that NAC administered simultaneously with liver damaging agent CCl4, exhibits not only protective, but also deleterious effects as indicated by several biochemical parameters.
N-乙酰-L-半胱氨酸（NAC）是一种常用于多种健康问题（如肝损伤）的药物。在某些情况下，已经出现了一些关于其负面影响的信息。我们研究的目的是检验其对长期负荷所致肝损伤的影响能力。我们通过四氯化碳诱导肝损伤（10周），并监测同时给予NAC（每日150毫克/千克体重）对肝脏形态和一些生化参数（三酰甘油、胆固醇、丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、胆红素、胆汁酸、蛋白质、白蛋白和胆碱酯酶）的影响。NAC显著降低了血浆中胆汁酸和胆红素的水平以及肝脏中三酰甘油的水平，所有这些指标在四氯化碳损伤后均升高。如胆固醇升高至对照水平所示，在NAC存在的情况下，四氯化碳诱导的胆固醇降低完全恢复。给予NAC并未改善组织学参数。除了NAC的保护作用外，我们还发现了其有害特性：同时给予四氯化碳和NAC会增加三酰甘油、ALT和AST活性，并显著增加血浆胆碱酯酶活性。我们观察到肝组织纤维化的百分比有非显著性增加。我们的结果表明，与肝损伤剂四氯化碳同时给予的NAC，从几个生化参数来看，不仅表现出保护作用，还表现出有害作用。
